AX-024 HCl can inhibit cytokine release especially the production of IL-6, TNFα, IFN-γ, IL-10 and IL-17A. It also modulates TCR signaling and prevents psoriasis and asthma.
AX-024 HCl 细胞实验
Cell Line
Concentration
Treated Time
Description
References
T lymphocytes
100 µM
24 h
Validate the specificity of AX-024 in inhibiting TCR-dependent T cell activation
J Neuroinflammation. 2025 Mar 13;22(1):78
monocytes
100 µM
24 h
Validate that AX-024 has no significant effect on inflammatory activation of non-T cell leukocytes (e.g., monocytes)
J Neuroinflammation. 2025 Mar 13;22(1):78
G8 γδ TCR-expressing Jurkat cells
10 nM
6 min
To test the effect of AX-024 on calcium signaling, results showed AX-024 did not affect the enhanced calcium response.
Front Immunol. 2018 Jul 9;9:1579
Jurkat Vγ9Vδ2 cells
10 nM
2 or 5 min
To test the effect of AX-024 on phosphorylation of ZAP70 and Erk in γδ T cells, results showed AX-024 did not affect phosphorylation levels.
Front Immunol. 2018 Jul 9;9:1579
human Jurkat T cells
1-6 nM
2.5 min
Inhibition of ZAP70 phosphorylation in residue Tyr-319, confirming the action of AX-024
J Biol Chem. 2020 Jul 17;295(29):10076
Primary CD4 T cells
20 µM
1 h
Evaluate the inhibitory effect of AX-024 on T cell proliferation
J Biol Chem. 2020 Jun 5;295(23):7849-7864
Jurkat T cells
10 nM
30 min
AX-024 inhibited Nck-CD3ε interaction, leading to increased lipid content in wild-type Jurkat T cells, similar to Nck1-deficient cells.
BMC Mol Cell Biol. 2022 Jul 28;23(1):36
AX-024 HCl 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6J male mice
Intracerebral hemorrhage (ICH) model (induced by collagenase or autologous blood injection)
Intraperitoneal injection
10 mg/kg
Starting at 1-hour post-ICH, once daily for 3 days
Inhibition of TCR activation alleviates neuroinflammation, brain edema, and white matter injury after ICH and improves neurobehavioral outcomes
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