There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
AEBSF directly inhibits β-secretase, thereby suppressing the constant production of Aβ across five diverse human cell lines, including both neural and non-neural types[1]. As a serine protease inhibitor, AEBSF prevents the lysis of leukemic cells by human macrophages without affecting the macrophage release of TNF-α and IL-1β[2]. AEBSF disrupts blastocyst development on endometrial cells and impedes the adhesion of HeLa cells to HUVECs by modifying the pattern of protein secretion[4].
体内研究
AEBSF administration (76.8 mg/kg daily, i.p.) extends the survival time of mice infected with a lethal T. gondii [3].
AEBSF also mitigates airway responsiveness and associated inflammation in a mouse model induced by cockroach allergens[4].
体外研究
AEBSF directly inhibits β-secretase, thereby suppressing the constant production of Aβ across five diverse human cell lines, including both neural and non-neural types[1].
As a serine protease inhibitor, AEBSF prevents the lysis of leukemic cells by human macrophages without affecting the macrophage release of TNF-α and IL-1β[2].
AEBSF disrupts blastocyst development on endometrial cells and impedes the adhesion of HeLa cells to HUVECs by modifying the pattern of protein secretion[4].
AEBSF HCl 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Neutrophils
1 mM
1 hour
Inhibition of IL-1β secretion
Front Immunol. 2018 Feb 19;9:269.
Human umbilical vein endothelial cells (HUVEC)
1 mg/ml
1 hour
Evaluate the uptake of PG-AF in HUVEC, showing that PG-AF accumulates in cells while free AF does not.
Int J Nanomedicine. 2017 Jan 13;12:577-591.
Human plasma
100 µg/mL
120 seconds
To assess the effect of AEBSF on the direct fibrinogen cleaving actions of C. mictlantecuhtli venom, results showed that AEBSF inhibited this action.
Front Immunol. 2021 Mar 11;12:612846.
Mono Mac 6 (MM6) cells
150 to 250 µM
18 hours
AEBSF blocked Dicer proteolysis, resulting in increased full-length Dicer and decreased 170 kDa and 50 kDa fragments.
Proc Natl Acad Sci U S A. 2020 Apr 14;117(15):8573-8583.
Human primary monocytes
250 µM
18 hours
AEBSF treatment resulted in strong up-regulation of full-length Dicer and reduced the 50 kDa fragment.
Proc Natl Acad Sci U S A. 2020 Apr 14;117(15):8573-8583.
Human retinal pigment epithelial cells (hRPE)
0-300 µM
2 hours
Pretreatment with AEBSF significantly inhibited TM-induced mitochondrial permeability transition and cell death in hRPE cells
Free Radic Biol Med. 2012 Sep 1;53(5):1111-22.
Keratinocytes
250 µM
20 hours
AEBSF significantly inhibited TLSP activity in the stratum corneum.
J Invest Dermatol. 2012 May;132(5):1435-42.
Uni-Graft prosthesis
0.6 mg/mL
3 hours
Inhibition of adhesion and multiplication of Staphylococcus aureus and Candida albicans
Biomolecules. 2020 Jan 4;10(1):82.
Human Umbilical Vein Endothelial Cells (HUVEC)
100 µM
4 hours
AEBSF inhibited the ATF6 signaling pathway, blocking the sFRP2-mediated angiogenesis through cTGF, resulting in reduced tubule formation in HUVECs.
Basic Res Cardiol. 2020 Jun 26;115(4):48.
AEBSF HCl 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
SFRP2 TG mice
Permanent Coronary Artery Occlusion Model
Osmotic Pump
2 μg/kg/day
Once daily for 1 week
AEBSF, by inhibiting the ATF6 signaling pathway, blocked the sFRP2-mediated cardioprotection and angiogenesis, resulting in the abolition of reduced infarct size and protection of cardiac function.
Basic Res Cardiol. 2020 Jun 26;115(4):48.
Mice
IL10-/- mice
Oral gavage
300 mg/kg
Starting from 1 day before V/M treatment and continuing during V/M treatment
To assess the effect of AEBSF on V/M-induced high protease activity and intestinal barrier function, results showed that AEBSF significantly reduced protease activity and protected intestinal barrier function.
Cell Mol Gastroenterol Hepatol. 2018 May 29;6(3):370-388.e3
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