8-Gingerol, a natural product isolated and purified from the rhizome of Zingiber officinale Roscoe. with anti-oxidant and anti-inflammatory activities, suppresses lipopolysaccharide (LPS) and concanavalin A (ConA)-stimulated splenocyte proliferation in vitro and cellular tyrosinase activity, inhibits the expression of MC1R, MITF, tyrosinase, TRP1 and TRP2, decreases melanin content, intracellular RS and ROS levels in B16F10 and B16F1 cells, inhibits melanogenesis by down-regulation of MAPK, PKA signaling pathway and it could be used as an effective skin-whitening agent. It also inhibits T lymphocyte proliferation and cytokine synthesis.
8-Gingerol/8-姜酚 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Human airway smooth muscle cells
100 μM
15 min
To investigate the effect of 8-Gingerol on acetylcholine-induced increases in intracellular calcium concentration. Results showed that 8-Gingerol significantly blunted the acetylcholine-induced calcium response.
Am J Respir Cell Mol Biol. 2013 Feb;48(2):157-63
ConA-stimulated mouse splenocytes
10, 20, 40, 80 μg/mL
44 h
To evaluate the effect of 8-Gingerol on ConA-stimulated splenocyte proliferation, showing significant inhibition only at 80 μg/mL
Molecules. 2011 Mar 22;16(3):2636-45
LPS-stimulated mouse splenocytes
10, 20, 40, 80 μg/mL
44 h
To evaluate the effect of 8-Gingerol on LPS-stimulated splenocyte proliferation, showing significant inhibition at 40 and 80 μg/mL
Molecules. 2011 Mar 22;16(3):2636-45
mouse splenocytes
0-100 μg/mL
24 h
To evaluate the effect of 8-Gingerol on splenocyte viability, showing no toxicity at concentrations up to 100 μg/mL
Molecules. 2011 Mar 22;16(3):2636-45
HepG2 cells
10 µM
48 h
To investigate the effect of 8-gingerol on CYP3A4 mRNA expression, results showed that 8-gingerol inhibited CYP3A4 mRNA expression by 58%±7%.
Acta Pharmacol Sin. 2013 Sep;34(9):1237-42
MV4-11 cells
10 μM
72 h
8GL treatment significantly upregulated the expression of CD11b and CD14, promoting MV4-11 cell differentiation.
Cell Death Dis. 2022 Nov 27;13(11):1004.
HL60 cells
10 μM
72 h
8GL treatment significantly upregulated the expression of CD11b and CD14, promoting HL60 cell differentiation.
Cell Death Dis. 2022 Nov 27;13(11):1004.
H9c2 cells
5, 10, 25μM
6 h
To evaluate the protective effect of 8-Gingerol on DOX-induced H9c2 cell injury. The results showed that 8-Gingerol significantly increased cell viability at concentrations of 5, 10, 25 μM.
Theranostics. 2023 Jan 1;13(2):510-530
8-Gingerol/8-姜酚 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
A/J mice
Airway hyperresponsiveness model
Nebulization
100 μM
Single dose, lasting 10 seconds
To investigate the effect of 8-Gingerol on methacholine-induced airway hyperresponsiveness. Results showed that 8-Gingerol significantly attenuated airway resistance.
Am J Respir Cell Mol Biol. 2013 Feb;48(2):157-63
Male Kunming mice
Isoproterenol-induced myocardial fibrosis model
Intraperitoneal injection
10 and 20 mg/kg
Once daily for 14 consecutive days
8-Gingerol significantly reduced J-point elevation and heart rate, decreased cardiac weight index and left ventricle weight index, serum levels of creatine kinase and lactate dehydrogenase (CK and LDH, respectively), ROS generation, and attenuated ISO-induced pathological heart damage. Moreover, 8-Gingerol significantly suppressed ISO-induced autophagosome formation, down-regulated the activities of matrix metalloproteinases-9 (MMP-9), Caspase-9, and Bax protein, up-regulated the activity of Bcl-2 protein, alleviated cardiomyocyte apoptosis, and modulated autophagy and apoptosis via the PI3K/Akt/mTOR signaling pathway.
Front Pharmacol. 2021 Jul 28;12:711701
BALB/c male mice
OVA-immunized mouse model
Intraperitoneal injection
25, 50, 100 mg/kg
Once daily for 7 consecutive days
To evaluate the immunosuppressive effect of 8-Gingerol in OVA-immunized mice, showing significant suppression of splenocyte proliferation and B cell percentage, and reduction of OVA-specific IgG, IgG1, and IgG2b levels at high doses (50, 100 mg/kg)
Molecules. 2011 Mar 22;16(3):2636-45
BALB/c nude mice
AML xenograft model
Intraperitoneal injection
30 mg/kg and 60 mg/kg
Once daily for 2 weeks
8GL significantly inhibited tumor growth and upregulated CD11b expression, promoting AML cell differentiation.
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