There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
γ-Oryzanol is a potent DNA methyltransferases (DNMTs) inhibitor in the striatum of mice. γ-Oryzanol significantly inhibits the activities of DNMT1 (IC50=3.2 μM), DNMT3a (IC50=22.3 μM). γ-oryzanol ameliorates HFD (high-fat diet)-induced DNA hypermethylation of the promoter region of D2R in the striatum of mice[1]. In pancreatic islets from both high-fat diet-induced and streptozotocin-induced diabetic mice, γ-oryzanol ameliorates endoplasmic reticulum stress and protects β-cells against apoptosis. γ-oryzanol also acts as a potent inhibitor against deoxyribonucleic acid methyltransferases in the brain reward system (striatum) in mice, thereby attenuating, at least partly, the preference for a high-fat diet through the epigenetic modulation of striatal dopamine D2 receptor[2]. γ-oryzanol modulates quantitative changes of proteins involved in synaptic plasticity and neuronal trafficking, neuroprotection and antioxidant activity, and mitochondria and energy metabolism[3]. Chronic consumption of γ-oryzanol can revert the LPS-induced cognitive and memory impairments by promoting hippocampal antioxidant and anti-inflammatory molecular responses[4].
To evaluate the effect of γ-Oryzanol on ER stress. γ-Oryzanol (1 mmol/L) significantly suppressed the tunicamycin-induced expression of ER stress-responsive genes (Chop, ERdj4, and Xbp1s).
Diabetes. 2012 Dec;61(12):3084-93.
HEK293 cells
10 μM
24 h
To evaluate the effect of γ-Oryzanol on the activities of ER stress-responsive cis-acting elements. γ-Oryzanol significantly suppressed the tunicamycin-induced activation of cis-acting elements (ERSE-I, 27%; ERSE-II, 64%; UPRE, 34% reduction vs. vehicle).
Promotes Neuronal Differentiation in Different In Vitro and In Vivo Models. Antioxidants (Basel).
3T3-L1 preadipocytes
30 μM
8 days
Orz promotes differentiation of 3T3-L1 preadipocytes by upregulating PPAR-γ and C/EBPα expression and increasing lipid accumulation.
Nutrients. 2015 Jun 15;7(6):4851-61.
γ-Oryzanol/γ-谷维素 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
Aging and ovariectomy-induced osteoporosis model
Intragastric administration
100 mg/kg
Daily for two months
To evaluate the protective effect of γ-Oryzanol on osteoporosis
Adv Sci (Weinh). 2021 Dec;8(24):e2100808
C57BL/6J mice
High-fat-diet-induced obesity model
Oral
320 μg/g
Continued for 12 weeks
To investigate the effects of γ-oryzanol on D2R signaling molecules in the striatum of HFD-induced obese mice. Results showed that γ-oryzanol decreased the expression and activity of DNMTs, restored the level of D2Rs in the striatum, and reduced the preference for HFD.
Diabetologia. 2017 Aug;60(8):1502-1511
C57BL/6J mice
High-fat diet-induced obesity model
Oral gavage
20, 80, 320 μg/g
Once daily for 13 weeks
Improved high-fat diet-induced islet dysfunction and enhanced glucose tolerance
Br J Pharmacol. 2015 Sep;172(18):4519-4534.
Ob/ob mice
Genetically obese-diabetic model
Oral
0.01 and 0.1 mg/g
Once every 2 weeks for 4 weeks
To evaluate the metabolic impact of γ-oryzanol nanoparticles in ob/ob mice. Results showed that Nano-Orz significantly improved glucose and lipid metabolism, reduced plasma insulin levels, and decreased ER stress and inflammation in liver and adipose tissue.
Drug Deliv. 2017 Nov;24(1):558-568
C57BL/6J mice
High-fat diet-induced obesity model
Oral gavage
20, 80, or 320 mg/kg
Once daily for 13 weeks
To evaluate the effects of γ-Oryzanol on glucose metabolism and hypothalamic ER stress. γ-Oryzanol significantly improved glucose intolerance and insulin sensitivity and reduced high-fat diet-induced hypothalamic ER stress.
Diabetes. 2012 Dec;61(12):3084-93.
Zebrafish
Nrf2a and nrf2b knockdown model
Microinjection in hindbrain ventricle
100 µg
Single injection, observed until 2 dpf
Promoted neuronal differentiation, increased neurog1-GFP signal and bdnf mRNA levels, dependent on Nrf2 pathway
Promotes Neuronal Differentiation in Different In Vitro and In Vivo Models. Antioxidants (Basel).
Mice
Adult mice
Oral gavage
100 mg/kg
Once a day for 21 days
Γ-Oryzanol improves cognitive function and modulates hippocampal proteome in mice
Nutrients. 2019 Mar 31;11(4):753
B6/129PF2 mice
Neuroinflammation model
Oral gavage
100 mg/kg
Once daily for 21 consecutive days
Γ-Oryzanol reverts LPS-induced cognitive and memory impairments by promoting hippocampal antioxidant and anti-inflammatory molecular responses.
Nutrients. 2019 Mar 29;11(4):728
Wistar rats
High-fat diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) model
Oral
0.16% (w/w) added to diets
Daily administration for 17 weeks
Γ-Oryzanol was able to reduce final body weights, fat weights, fasting glucose, fasting insulin, serum, and serum levels of liver function enzymes as well as the inflammatory markers such as TNF-α, IL-6, and leptin in HFD-fed rats. It also improved hepatic structure and glucose tolerance, increased adiponectin levels, and reduced serum and hepatic levels of TGs and CHOL in these rats. These effects were concomitant with a reduction in hepatic MDA and serum LDL-C levels, but also with an increment in hepatic SOD and GSH levels.
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