Plantamajoside (PMS) is a phenylpropanoid glycoside isolated from Plantago asiatica L.(Plantaginaceae). Plantamajoside ameliorated the lung injury that was induced by LPS. Plantamajoside suppressed the production of IL-1β, IL-6 and TNF-α in a dose-dependent manner. Plantamajoside inhibited the phosphorylation of IκBα, p65, p38, JNK and ERK. Plantamajoside has protective effect on LPS-induced ALI (acute lung injury) in mice and in RAW264.7 cells[3]. In vitro, PMS inhibited the ISO(isoproterenol)-induced increase in H9c2 cell surface area and the mRNA expression of ANP, BNP and Myh7. In vivo, PMS improved the ISO-induced decrease in cardiac function, inhibited the increase in cardiac anatomical parameters and alleviated the histopathological changes in cardiac tissues[4]. PMS inhibited the invasion, migration and viability of malignant melanoma cells. In addition, PMS induced apoptosis by regulating the expression levels of apoptotic-related genes and the activation of the PI3K/AKT signaling pathway, thereby exerting anti-malignant melanoma effects[5]. Plantamajoside decreased the production of PGE2, NO, IL-6, and IL-8 in LPS-stimulated HGFs. LPS-induced NF-κB p65 and IκB phosphorylation were also suppressed by plantamajoside. Furthermore, plantamajoside inhibited LPS-induced PI3K and AKT phosphorylation[6]. PMS protected against myocardial I/R injury via attenuating oxidative stress, inflammatory response and apoptosis[7].
plantamajoside/大车前苷 细胞实验
Cell Line
Concentration
Treated Time
Description
References
4T1 mouse breast cancer cell line
31.25, 62.5, 125, 250, 500 μg/mL
0, 12, 24, 36, 48 hours
To evaluate the effect of PMS on cell proliferation, results showed that PMS significantly inhibited the proliferation of 4T1 cells in a dose- and time-dependent manner
BMC Cancer. 2015 Dec 16;15:965
MDA-MB-231 human breast cancer cell line
31.25, 62.5, 125, 250, 500 μg/mL
0, 12, 24, 36, 48 hours
To evaluate the effect of PMS on cell proliferation, results showed that PMS significantly inhibited the proliferation of MDA-MB-231 cells in a dose- and time-dependent manner
BMC Cancer. 2015 Dec 16;15:965
MIN6 cells
12.5 µM, 25 µM, 50 µM
24 hours
To evaluate the protective effect of PMS on high glucose and palmitic acid-induced MIN6 cell damage. PMS intervention improved cell injury, reduced intracellular ROS levels, decreased LDH activity, and increased insulin secretion.
World J Diabetes. 2025 Feb 15;16(2):99053
RSC96 cells
12.5, 25, 50 µM
24 hours
To evaluate the protective effect of PMS on high glucose-induced RSC96 cell damage. Results showed that PMS enhanced cell viability and reduced LDH activity in a concentration-dependent manner.
J Cell Mol Med. 2025 Apr;29(8):e70571
Chinese Hamster Ovary (CHO-K1) cells
300 μg/mL
36 hours
To evaluate the side effects of PMS on normal cells, results showed that PMS had no significant effect on CHO cell viability
BMC Cancer. 2015 Dec 16;15:965
H9c2 myocardial cells
5-40 µM
48 hours
PMS was not cytotoxic in the concentration range of 5–40 μM and increased cell survival after H/R injury in a concentration-dependent manner without affecting proliferation or growth.
BMC Complement Med Ther. 2023 Feb 24;23(1):64
HepG2/SOR cells
200 ng/mL
48 hours
To evaluate the anti-cancer effect of PMS and SOR on HepG2/SOR cells. Results showed that PMS significantly enhanced the cytotoxicity of SOR to drug-resistant HepG2/SOR cells.
Drug Deliv. 2019 Dec;26(1):1080-1091
MCF-12A
400 µM
72 hours
Evaluation of the cytotoxic effect of plantamajoside on MCF-12A cells, showing an IC50 value of 296.2 μM
Life (Basel). 2023 Feb 16;13(2):556
HepG2
300 µM
72 hours
Evaluation of the cytotoxic effect of plantamajoside on HepG2 cells, showing an IC50 value of 156.1 μM
Life (Basel). 2023 Feb 16;13(2):556
U138-MG
300 µM
72 hours
Evaluation of the cytotoxic effect of plantamajoside on U138-MG cells, showing an IC50 value of 266.7 μM
Life (Basel). 2023 Feb 16;13(2):556
OVCAR-3
200 µM
72 hours
Evaluation of the cytotoxic effect of plantamajoside on OVCAR-3 cells, showing an IC50 value of 138.9 μM
Life (Basel). 2023 Feb 16;13(2):556
MDA-MB-231
300 µM
72 hours
Evaluation of the cytotoxic effect of plantamajoside on MDA-MB-231 cells, showing an IC50 value of 263.1 μM
Life (Basel). 2023 Feb 16;13(2):556
MCF-7
300 µM
72 hours
Evaluation of the cytotoxic effect of plantamajoside on MCF-7 cells, showing an IC50 value of 170.8 μM
Life (Basel). 2023 Feb 16;13(2):556
plantamajoside/大车前苷 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Sprague Dawley rats
High-fat diet-induced NAFLD model
Intraperitoneal injection
20, 40, 80 mg/kg
Once daily for 4 weeks
PMS alleviated liver damage, improved immune dysregulation and abnormal hepatic lipid metabolism in HFD-induced NAFLD rats by activating the AMPK/Nrf2 pathway
Kaohsiung J Med Sci. 2023 Aug;39(8):801-810
BALB/c mice
4T1 cell-induced allograft mammary tumor model
Oral administration
200 mg/kg
Once daily for 21 consecutive days
To evaluate the effect of PMS on tumor growth and pulmonary metastasis in vivo, results showed that PMS significantly reduced allograft tumor volume and weights, significantly decreased microvascular density and significantly lowered lung metastasis rate
BMC Cancer. 2015 Dec 16;15:965
C57BL/6 male mice
Acute sepsis model induced by cecal ligation and puncture (CLP)
Intraperitoneal injection
25, 50, 100 mg PMS/kg
Assessed within 24 hours after modeling and treatment
PMS alleviated sepsis-induced organ dysfunction by inhibiting the TRAF6/NF-κB axis, improved survival rates, reduced lung, liver, and heart injuries, and suppressed inflammatory responses and apoptosis
Pharm Biol. 2023 Dec;61(1):897-906
C57BL/6 mice
Diabetic peripheral neuropathy (DPN) model
Oral
25, 50, 100 mg/kg
Once daily for 4 weeks
To evaluate the therapeutic effect of PMS on DPN mice. Results showed that PMS improved mechanical pain threshold, thermal pain reaction time, and nerve conduction velocity, and reduced pathological damage to the sciatic nerve.
J Cell Mol Med. 2025 Apr;29(8):e70571
Spontaneously hypertensive rats (SHR)
Spontaneously hypertensive rat model
Oral
400 mg/kg
Once daily for 12 weeks
To investigate the antihypertensive effect and underlying mechanisms of PASE in SHR. Results showed PASE significantly lowered blood pressure and improved cardiac and aortic indices and collagen accumulation.
Front Pharmacol. 2019 Apr 30;10:403
C57BL/6 mice
Type 2 diabetes model
Oral gavage
50 mg/kg and 100 mg/kg
Once daily for 4 weeks
To evaluate the therapeutic effects of PMS on type 2 diabetes mice. PMS intervention significantly improved body weight, fasting blood glucose, and glycated serum protein levels, and alleviated pancreatic tissue damage.
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