There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
iCRT3 is an inhibitor of both Wnt and β-catenin-responsive transcription.
iCRT3 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HCT116 cells
50 µM
1 day
To validate the inhibitory effect of iCRT3 on Wnt target genes, iCRT3 significantly inhibited AXN2 and CYCD1 mRNA expression
Proc Natl Acad Sci U S A. 2011 Apr 12;108(15):5954-63.
Mouse embryonic stem cells (mESCs)
10 µM
14 days
ICRT3 treatment enhanced the expression of pluripotency markers and reduced the expression of differentiation markers in mESCs.
J Cell Biol. 2015 Oct 12;211(1):39-51.
HCMEC/D3
20 µM
15 hours
ICRT3 inhibited the binding of β-catenin to the transcription factor TCF-4 in hCMEC/D3 cells, resulting in significant reductions in the mRNA expression of claudin-3, claudin-5, Pgp, and BCRP, and decreased monolayer electrical impedance, indicating reduced tight junction integrity.
Sci Rep. 2019 Dec 23;9(1):19718.
Drosophila Cl8 cells
9 ng/μL
16 hours
To screen for inhibitors of the Wnt/β-catenin signaling pathway, iCRT3 significantly inhibited dTF12 reporter activity
Proc Natl Acad Sci U S A. 2011 Apr 12;108(15):5954-63.
A549 cells
5 µM
24 hours
ICRT3 significantly decreased the expression of LH3, Wnt1, β-catenin, and p-Smad3 proteins, and reduced collagen pyridine-crosslinking production.
Arch Med Sci. 2019 Jan 16;16(2):436-445.
Mouse embryonic stem cells (mESCs)
10 µM
48 hours
ICRT3 treatment reduced RA-induced differentiation and enhanced the expression of pluripotency markers.
J Cell Biol. 2015 Oct 12;211(1):39-51.
C1.1 cells
10 µM
48 hours
To investigate the effect of iCRT3 on TGF-β-induced cell migration and collagen expression. Results showed that iCRT3 combined with TGF-β significantly increased β-catenin/Foxo interaction, reduced β-catenin/TCF interaction, and decreased TGF-β-induced collagen expression.
Am J Pathol. 2021 Jun;191(6):993-1009.
PLC/PRF/5 cells
200 µM
48 hours
ICRT3 repressed the protein levels of TCF1, Survivin and PRC1
Mol Cells. 2018 Sep 30;41(9):830-841.
BT-549
25, 50, 75 µM
48 hours
ICRT-3 effectively inhibits cell proliferation in BT-549 cells in a dose- and time-dependent manner.
J Transl Med. 2013 Nov 4;11:280.
MDA-MB-231
25, 50, 75 µM
48 hours
ICRT-3 effectively inhibits cell proliferation in MDA-MB-231 cells in a dose- and time-dependent manner.
J Transl Med. 2013 Nov 4;11:280.
HCC-1143
25, 50, 75 µM
48 hours
ICRT-3 effectively inhibits cell proliferation in HCC-1143 cells in a dose- and time-dependent manner.
J Transl Med. 2013 Nov 4;11:280.
HCC-1937
25, 50, 75 µM
48 hours
ICRT-3 effectively inhibits cell proliferation in HCC-1937 cells in a dose- and time-dependent manner.
J Transl Med. 2013 Nov 4;11:280.
RAW264.7 macrophages
12.5, 25, 50, 75 µM
50 minutes pretreatment followed by 4 hours LPS stimulation
ICRT3 significantly reduces LPS-induced Wnt/β-catenin activity and inhibits TNF-α production and IκB degradation in a dose-dependent manner.
Sci Rep. 2017 Aug 23;7(1):9235.
HEK293 cells
25 µM
To validate the inhibitory effect of iCRT3 on the Wnt signaling pathway, iCRT3 significantly inhibited STF16 reporter activity
Proc Natl Acad Sci U S A. 2011 Apr 12;108(15):5954-63.
iCRT3 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6 mice
Unilateral renal ischemia reperfusion injury model
Intraperitoneal injection
10 mg/kg
Daily for 7 or 14 days
To investigate the protective effect of iCRT3 on renal ischemia-reperfusion injury. Results showed that iCRT3 combined with TGF-β significantly increased β-catenin/Foxo interaction, reduced kidney fibrosis, and promoted tubular epithelial repair.
Am J Pathol. 2021 Jun;191(6):993-1009.
C57BL/6 mice
Cecal ligation and puncture (CLP)-induced sepsis model
Intraperitoneal injection
5 and 10 mg/kg
Single dose, administered 5 hours after CLP
ICRT3 treatment significantly reduces plasma levels of proinflammatory cytokines and organ injury markers, and improves histological integrity of lung tissue, reducing lung collagen deposition and apoptosis.
Sci Rep. 2017 Aug 23;7(1):9235.
Nude mice
HCT116 and HT29 xenograft models
Intraperitoneal injection
50 mg/kg
Three times a week for 3 weeks
To validate the anti-tumor effect of iCRT14 in vivo, iCRT14 significantly inhibited tumor growth
Proc Natl Acad Sci U S A. 2011 Apr 12;108(15):5954-63.
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