Usnic acid dose-dependently increased ROS levels in H520 cells
Int J Mol Sci. 2020 Jan 29;21(3):876
HCT116 cells
5 µM
24 hours
Suppressed cell invasion
JACS Au. 2024 Apr 11;4(4):1521-1537
Streptococcus mutans UA 159
0.48 to 250 μg/ml
1 min
To evaluate the anti-acidogenic and anti-aciduric effects of usnic acid on S. mutans biofilms. Results showed significant inhibition of acid production and ATPase activity in biofilm cells.
Biofilm. 2024 Nov 30;8:100241
Ca2+- or Zn2+-loaded liposomes
20 µM or 40 µM
10 minutes
Measure the efflux of Ca2+ or Zn2+ induced by usnic acid. Results showed Zn2+ efflux was faster than Ca2+.
Int J Mol Sci. 2022 Dec 19;23(24):16203
Calu-1 cells
30 µM
12 hours
Usnic acid dose-dependently increased ROS levels in Calu-1 cells
Int J Mol Sci. 2020 Jan 29;21(3):876
Sporozoites
30 µM
2 hours
Induced apoptosis in sporozoites and lowered mitochondrial membrane potential
J Anim Sci Biotechnol. 2025 May 12;16(1):67
Human blood cells
125 μg/mL
24 hours
Evaluate the cytotoxic effect of usnic acid on human blood cells, showing a significant decrease in cell viability and induction of early apoptosis
Antioxidants (Basel). 2021 Jul 23;10(8):1171
Human blood cells
75 μg/mL
24 hours
Evaluate the cytotoxic effect of usnic acid on human blood cells, showing a significant decrease in cell viability and induction of early apoptosis
Antioxidants (Basel). 2021 Jul 23;10(8):1171
Human blood cells
50 μg/mL
24 hours
Evaluate the cytotoxic effect of usnic acid on human blood cells, showing a significant decrease in cell viability
Antioxidants (Basel). 2021 Jul 23;10(8):1171
Human blood cells
25 μg/mL
24 hours
Evaluate the cytotoxic effect of usnic acid on human blood cells, showing a slight decrease in cell viability
Antioxidants (Basel). 2021 Jul 23;10(8):1171
Mycobacterium tuberculosis H37Ra
512 μg/ml
24 hours
To evaluate the effect of usnic acid on Mycobacterium tuberculosis at transcriptomic and metabolomic levels, results showed that usnic acid treatment provoked disturbances of redox state in mycobacterial cells and increased production of structural elements of the cell wall and cell membrane
mSystems. 2021 May 4;6(3):e00097-21
Calu-1 cells
10 to 40 µM
24 hours
Usnic acid dose-dependently induced apoptosis in Calu-1 cells
Int J Mol Sci. 2020 Jan 29;21(3):876
H520 cells
10 to 40 µM
24 hours
Usnic acid dose-dependently induced apoptosis in H520 cells
Int J Mol Sci. 2020 Jan 29;21(3):876
HepG2 cells
3.13-50 µM
24 hours
To investigate the toxic effects of usnic acid on HepG2 cells, including DNA damage, S-phase cell cycle arrest, and oxidative stress. Results showed that usnic acid caused DNA damage and S-phase cell cycle arrest within 24 hours and triggered oxidative stress.
Arch Toxicol. 2017 Mar;91(3):1293-1307
Yeast cells
40 µM
2-5 minutes
To observe the effect of Usnic acid on nuclear accumulation of Rpl7a-GFP, results showed Rpl7a-GFP accumulated in the nucleus within 2 minutes.
Nat Commun. 2024 Aug 29;15(1):7511
HNEpC cells
75 mcg/mL
3 hours
Evaluate the blocking effect of UA on VSV-based pseudovirus SARS-CoV-2 infection, results showed UA effectively blocked viral infection.
Int J Mol Sci. 2023 Feb 12;24(4):3695
Vero E6 cells
75 mcg/mL
3 hours
Evaluate the blocking effect of UA on VSV-based pseudovirus SARS-CoV-2 infection, results showed UA effectively blocked viral infection.
Int J Mol Sci. 2023 Feb 12;24(4):3695
Yeast cells
40 µM
30 minutes
To observe the effect of Usnic acid on nucleolar accumulation of Rps9-GFP, results showed Rps9-GFP accumulated in the nucleolus within 30 minutes.
Nat Commun. 2024 Aug 29;15(1):7511
Coccidian oocysts
30 µM
8 hours
Induced apoptosis in coccidian oocysts, lowered mitochondrial membrane potential, and disrupted oocyst structure
J Anim Sci Biotechnol. 2025 May 12;16(1):67
Usnic acid/松萝酸 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Caenorhabditis elegans
Wild-type animals and various mutants
Administered via culture medium
2 mM
Continuous administration starting from L4 larval stage
Usnic Acid extended healthspan and improved neurodegeneration diseases by inhibiting the mTOR/PHA-4 signaling pathway
iScience. 2022 Nov 9;25(12):105539
Broiler chickens
Necrotic enteritis model
Oral
300 mg/kg
Once daily for 42 days
Improved growth performance, reduced intestinal lesion scores, decreased fecal coccidian oocyst counts, and enhanced intestinal barrier function and immune response
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