Trolox is a water-soluble analogue of vitamin E with a powerful antioxidant effect. In a canine model of two hours of left anterior descending coronary artery occlusion followed by four hours of reperfusion, Trolox and ascorbic acid reduced the area of infarction within the area at risk. Trolox has shown to protect mammalian cells from oxidative damage[1]. Trolox could prevent oxidative stress-induced apoptosis in thymocytes. Pre- or posttreatment of cells with Trolox reduced H2O2-induced DNA fragmentation to control levels and below[2]. Trolox prolonged substantially the survival of human ventricular myocytes and hepatocyte against oxyradicals generated with xanthine oxidase plus hypoxanthine, and prevented lysis of red cells exposed to an azo-initiator (2,2'-azo-bis(2-amidinopropane) HCl). In each cell type, the protection by Trolox was dose dependent and surpassed those given by such water-soluble antioxidants as ascorbic acid, superoxide dismutase, and (or) catalase, each examined at or near its optimal level in the same system[3]. Trolox treatment in BDL (bile duct ligation) rats prevented the increase in ALT(Alanine aminotransferase). Collagen was increased by chronic BDL, but trolox administration preserved the normal collagen concentration[4]. Trolox prevents osteoclast formation and bone loss by inhibiting both RANKL induction in osteoblasts and c-Fos expression in osteoclast precursors[5].
Trolox/水溶性维生素E 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HeLa cells
2.5-160 µM
2 hours
To study the dual antioxidant/prooxidant behavior of Trolox within 2 hours, results showed antioxidant activity at low concentrations (2.5-20 µM) and prooxidant effect at high concentrations (40-160 µM), inducing apoptotic volume decrease (AVD).
Antioxidants (Basel). 2020 Oct 29;9(11):1058.
HeLa cells
2.5-160 µM
24 hours
To study the dual antioxidant/prooxidant behavior of Trolox over a wide range of concentrations, results showed antioxidant activity at low concentrations (2.5-15 µM) and prooxidant effect at high concentrations (40-160 µM), inducing apoptosis.
Antioxidants (Basel). 2020 Oct 29;9(11):1058.
Human sperm
5 mM and 10 mM
45 minutes and 90 minutes
5 mmol/L trolox protected the Wi-Fi-exposed semen in vitro from the damage of electromagnetic radiation-induced oxidative stress.
Chin Med J (Engl). 2018 Feb 20;131(4):402-412.
Trolox/水溶性维生素E 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Rats
Hepatic ischemia/reperfusion model
Intravenous injection
2.5mg/kg
Single dose 5 minutes before reperfusion
To investigate the effect of Trolox on hepatic microsomal cytochrome P450 (CYP) activity and gene expression during ischemia and reperfusion (I/R). Results showed that Trolox attenuated liver injury, restored CYP content and NADPH-cytochrome P450 reductase activity, prevented the decrease in CYP1A1, CYP1A2, and CYP2B1 activities, and inhibited the increase in CYP2E1 activity.
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