Sterile PBS or 0.9% saline for reconstitution/dilution. It is recommended to use the reconstituted/diluted product within one month.
Tocilizumab/托珠单抗 细胞实验
Cell Line
Concentration
Treated Time
Description
References
H1467 primary human chondrocytes
1.0 µg/mL
24 hours
To evaluate the preventive and therapeutic effects of Tocilizumab on H2O2-induced chondrocyte damage. Results showed that Tocilizumab significantly reduced IL-6 and COX-2 expression and further enhanced anti-inflammatory effects in the combination therapy group.
Biomolecules. 2024 Dec 20;14(12):1636
BL1 TNBC cell lines (MDA-MB-468, HCC38)
30 ng/ml
48 hours
Evaluate the effect of Tocilizumab on IL-6 signaling in BL1 TNBC cell lines, results showed inconsistent reduction of STAT3 phosphorylation.
NPJ Breast Cancer. 2022 Mar 8;8(1):30
MSL TNBC cell lines (SUM-159, MDA-MB-436)
30 ng/ml
48 hours
Evaluate the effect of Tocilizumab on IL-6 signaling in MSL TNBC cell lines, results showed Tocilizumab significantly reduced STAT3 phosphorylation.
NPJ Breast Cancer. 2022 Mar 8;8(1):30
RA synoviocytes and healthy PBMC
10 and 100 μg/ml
48 hours
To evaluate the effect of Tocilizumab on cytokine production. Tocilizumab significantly inhibited the production of IL-17 and IL-1β but had no significant effect on IL-6 secretion.
Front Immunol. 2016 Nov 17;7:509
Tocilizumab/托珠单抗 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
NU/NU mice
Human colorectal carcinoma xenograft model
Peritoneal injection
1 mg/kg
Three times a week for 11 days
Inhibited tumor growth in SW480 xenografts but showed minimal effect on HT-29 xenografts
Pharmaceuticals (Basel). 2024 Jan 18;17(1):127
Female NSG mice
MSL TNBC xenograft models (MDA-MB-436, SUM-159)
Intraperitoneal injection
20 mg/kg
Once every 2 weeks for five cycles
Evaluate the efficacy of Tocilizumab combined with docetaxel in MSL TNBC xenograft models, results showed combination therapy significantly delayed tumor progression and improved survival.
NPJ Breast Cancer. 2022 Mar 8;8(1):30
Nude mice
U251 cell xenograft model
Caudal vein injection
8 mg/kg/month
Monthly for 30 days
Tocilizumab significantly inhibited tumor growth and reduced IL6, p-STAT3, and LC3B expression
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