Deoxycholic acid sodium salt is specifically responsible for activating the G protein-coupled bile acid receptor TGR5 that stimulates brown adipose tissue (BAT) thermogenic activity[3]. Deoxycholic acid (DCA) and chenoDeoxycholic acid (CDCA), as the common ingredients of duodenal reflux, act synergistically in many physiological and pathological processes. To simulate chronic local recurrent disease in vitro, the gastric cancer cell line MGC803 is exposed to acidified medium (pH 5.5) containing 100 μM Deoxycholic acid and CDCA. An untreated log-growth MGC803 cell line is generated to be used as a control in normal pH media. After daily 10 min exposure to the acidified bile acids for 60 weeks, MGC803-resistant cells are able to survive and proliferate after 120 min exposure[2].
Sodium Deoxycholate/脱氧胆酸钠 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
Hypercholesterolemia model
Tail vein injection
0.75 mg/kg
Once a week for 1 month
To evaluate the gene silencing effect of Toc-HDO in a hypercholesterolemia model, results showed that Toc-HDO significantly reduced ApoB mRNA levels in the liver and LDL cholesterol levels in serum.
Nat Commun. 2015 Aug 10;6:7969
Mice
Collagen-induced arthritis model
Subcutaneous injection
100 µg/mouse
Single injection, observed for 21 days
To evaluate the role of Deoxycholate in the arthritis model, the results showed that the development of arthritis led to a significant increase in plasma levels of TNF-α and leptin, accompanied by a systemic insulin resistance state.
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