Skimmianin, a natural product isolated and purified from the herbs of Skimmia japonica, is an inhibitor against the Leishmania APRT enzyme and a strong acetylcholinesterase (AChE) inhibitor, and has a selective inhibitory effect on the 5-hydroxytryptamine-induced vasopressor responses of rats. Skimmianine also has anti-inflammatory and leishmanicidal effect, thus it is a promising drug candidate for L. braziliensis due to its potent immunomodulatory activity.
Skimmianine/茵芋碱 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HepG2.2.15 cells
10 nM to 10 µM
13 days
To assess the effect of Skimmianine on HBV replication, results showed Skimmianine did not significantly inhibit HBV replication
Viruses. 2024 Aug 22;16(8):1346
HT-22 neuronal cells
10, 20, 30 µM
24 hours
To evaluate the protective effects of skimmianine against neurotoxicity induced by microglia-conditioned media. Results showed that Skimmianine pre-treatment protected HT-22 cells from toxicity induced by microglia-conditioned media, as well as increasing MAP-2 expression.
Molecules. 2023 Jan 30;28(3):1317
BV-2 microglia cells
10, 20, 30 µM
24 hours
To evaluate the effects of skimmianine on the production of pro-inflammatory mediators in LPS-activated BV-2 microglia. Results showed that Skimmianine reduced LPS-induced elevated secretion of TNF α, IL-6, NO, and PGE2, as well as the increased protein expression of iNOS and COX-2.
Molecules. 2023 Jan 30;28(3):1317
AML12 cells
40 µM
24 hours
To evaluate the protective effect of Skimmianine on hypoxia-reoxygenation (H/R) injury in AML12 cells. The results showed that Skimmianine significantly reduced cell injury and apoptosis caused by H/R.
Sci Rep. 2023 Oct 25;13(1):18232
PXB cells
100 pM to 10 µM
34 days
To evaluate the inhibitory effect of Skimmianine on HBV infection, results showed Skimmianine significantly inhibited HiBiT activity at low concentrations
Viruses. 2024 Aug 22;16(8):1346
Skimmianine/茵芋碱 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Rats
Sprague-Dawley rats
Oral
20 mg/kg
Single dose
To investigate the metabolic profile of skimmianine in rats and identify its metabolites
Molecules. 2017 Mar 23;22(4):489
Female Wistar albino rats
DMBA-induced breast cancer model
Intraperitoneal injection
40 mg/kg
Once weekly for four weeks
To evaluate the antitumor and immunomodulatory potential of Skimmianine in breast cancer. Results showed that Skimmianine significantly reduced tumor burden and decreased the expression levels of CA15-3, PCNA, and TNF-α.
Pharmaceuticals (Basel). 2025 May 20;18(5):756
C57BL/6J mice
Liver ischemia/reperfusion (I/R) injury model
Gavage
40 mg/kg
Once daily for 14 days
To evaluate the protective effect of Skimmianine on liver I/R injury. The results showed that Skimmianine significantly reduced serum transaminase levels, liver necrosis area, oxidative stress, and apoptosis levels.
搜索
