To detect fusion protein expression levels by immunoblotting
Cell. 2006 Sep 8;126(5):995-1004
HEK-pNF κB-DD-tdTomato-C8
1 µM
18 hours
To study the stabilizing effect of Shield-1 on NF-κB-dependent DD-tdTomato expression, results showed that Shield-1 significantly enhanced the fluorescence signal of DD-tdTomato.
Int J Mol Sci. 2021 Dec 16;22(24):13530
NIH3T3 cells
3.3 nM (IC50)
24 hours
To evaluate the degradation effect of Shield-1 on YFP-LID fusion protein, results showed that Shield-1 induced YFP degradation in a dose-dependent manner.
Nat Chem Biol. 2011 Jul 3;7(8):531-7
NIH3T3 cells
1 µM
24 hours
To evaluate the effect of Shield-1 on gene expression in NIH3T3 cells, results showed that Shield-1 at 1 μM concentration had only minor effects on a few genes.
J Biol Chem. 2007 Aug 24;282(34):24866-72
HeLa Kb cells
5 µM
4 hours
Compare antigen presentation efficiency between transfected and virus-infected SCRAP, with transfected cells showing ~8.3-fold higher efficiency
Proc Natl Acad Sci U S A. 2012 May 1;109(18):7025-30
EL4 cells
5 µM
4 hours
Measure the efficiency of generating Kb–SIINFEKL complexes from nascent vs. retired proteins, showing ∼2% efficiency
Proc Natl Acad Sci U S A. 2012 May 1;109(18):7025-30
Human newborn foreskin fibroblasts (HFFs)
1 µM
48 hours
Shield-1 was used to stabilize the ddFKBP-tagged UL79 protein, allowing the replication of the recombinant virus AD ddUL79. In the absence of Shield-1, AD ddUL79 failed to replicate, indicating the essential role of UL79 in HCMV replication.
J Virol. 2011 May;85(10):4841-52
Human foreskin fibroblasts (HFF)
1 µM
48 hours
To stabilize the UL51-FKBP fusion protein and prevent its degradation
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