Sanguinarine is a benzophenanthridine alkaloid which has anti-microbial, anti-oxidant and anti-inflammatory properties. It is a specific inhibitor of Rac1b, and with potential antitumor activity.
Sanguinarine/血根碱 细胞实验
Cell Line
Concentration
Treated Time
Description
References
MDA-MB-468 cells
2.0–5.0 µM
24 hours
To evaluate the cytotoxic effects of SANG on TNF-α-activated TNBC cells, results showed significant inhibition of cell viability
Int J Mol Sci. 2022 Jul 28;23(15):8329.
MDA-MB-231 cells
2.0–5.0 µM
24 hours
To evaluate the cytotoxic effects of SANG on TNF-α-activated TNBC cells, results showed significant inhibition of cell viability
Int J Mol Sci. 2022 Jul 28;23(15):8329.
HEK293T cells
1 µM
4 days
To evaluate the effect of sanguinarine on the proliferation of Rac1b cells, results showed that sanguinarine inhibited the proliferation of Rac1b cells, accompanied by significantly increased levels of PARP-89, and decreased levels of cyclin-D1 and the percentage of BrdU positive cells.
Acta Pharmacol Sin. 2015 Feb;36(2):229-40.
LX-2 cells
0.75 µM, 1.50 µM, 3.00 µM
48 hours
To evaluate the toxicity of compounds 1 and 1a on LX-2 cells. The results showed that 1a was less cytotoxic than 1 to normal LX-2 hepatic stellate cells.
Int J Mol Sci. 2023 Apr 30;24(9):8113.
HEL cells
0.75 µM, 1.50 µM, 3.00 µM
48 hours
To evaluate the inhibitory effects of compounds 1 and 1a on the proliferation of HEL cells. The results showed that compounds 1 and 1a inhibited HEL cell proliferation in a dose-dependent manner.
Int J Mol Sci. 2023 Apr 30;24(9):8113.
Caco-2 cells
0.5 μM
Decreased the number of SARS-CoV-2 genome copies
Cell Mol Immunol. 2023 Dec;20(12):1457-1471.
HeLa cells
0.5, 1, 2 μM
Inhibited CD97 expression, reduced VSV-G expression levels and virus titer
Cell Mol Immunol. 2023 Dec;20(12):1457-1471.
LoVo cells
0, 1, 2, 4 µM
48 h
To evaluate the effect of SG on the migration ability of LoVo cells, results showed that SG significantly reduced the number of migrated cells.
Clin Transl Med. 2020 Jan;10(1):1-12.
Caco-2 cells
0.39-50 µM
48 h
To evaluate the anticancer effect of SG on CRC cells, results showed that SG had an IC50 value of 5.20 µM for Caco-2 cells.
Clin Transl Med. 2020 Jan;10(1):1-12.
SW480 cells
0.39-50 µM
48 h
To evaluate the anticancer effect of SG on CRC cells, results showed that SG had an IC50 value of 5.37 µM for SW480 cells.
Clin Transl Med. 2020 Jan;10(1):1-12.
LoVo cells
0.39-50 µM
48 h
To evaluate the anticancer effect of SG on CRC cells, results showed that SG had a selective inhibitory effect on LoVo cells with an IC50 value of 4.03 µM.
Clin Transl Med. 2020 Jan;10(1):1-12.
HEK293T cells
1 µM
4 days
To evaluate the effect of Sanguinarine on the proliferation of Rac1b cells, results showed that Sanguinarine inhibited the proliferation of Rac1b cells, accompanied by significantly increased levels of PARP-89, and decreased levels of cyclin-D1 and the percentage of BrdU positive cells.
Acta Pharmacol Sin. 2015 Feb;36(2):229-40.
Rabbit muscle SR vesicles
10-20 μM
To study the effects of sanguinarine on Ca2+ release from SR vesicles, results showed that sanguinarine induced Ca2+ release, which could be blocked by RR and DTT
Br J Pharmacol. 2000 May;130(2):299-306.
Mouse diaphragm
40 μM
To investigate the direct effects of sanguinarine on muscle contractility, results showed that sanguinarine induced dose-dependent muscle contracture
Br J Pharmacol. 2000 May;130(2):299-306.
Sanguinarine/血根碱 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Nude mice
HepG2 and SMMC-7721 cell xenograft models
Oral
1.25, 2.5, 5 mg/kg
Consecutive 3 days daily administration, followed by every other day, total 10 times
To evaluate the inhibitory effect of Sanguinarine on tumor growth, results showed that Sanguinarine significantly inhibited tumor growth.
Cell Death Dis. 2019 Dec 9;10(12):939
Nude mice
LoVo cells xenograft model
Oral
1.25, 2.5, 5 mg/kg
Once daily for 14 days
To evaluate the inhibitory effect of SG on the growth of LoVo cell xenograft tumors, results showed that SG significantly inhibited tumor growth with inhibitory rates of 47.12%, 58.65%, and 68.67%.
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