SMIFH2 is a specific inhibitor of formins, a group of proteins that mediate actin polymerization and consequently influence the actin cytoskeleton structure[1].
体外研究
When administered to U2OS cells at 25 μM for periods ranging from 1 to 16 hours, SMIFH2 triggers dynamic cytoskeletal remodeling, showcasing its direct impact on cellular architecture[1].
Additionally, SMIFH2 at the concentration of 25 μM leads to a reduction in the levels of p300, mDia2, and p53 in a proteasome-independent manner, illustrating its broad biological effects, including potential modulation of protein stability and cell signaling pathways[1].
SMIFH2 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Cos-7 cells
40 µM
1-2 hours
To investigate the effect of formin inhibitor SMIFH2 on filopodia adhesion, results showed that SMIFH2 treatment caused filopodia to detach from the beads, significantly shortening the contact time and weakening the exerted force.
Nat Commun. 2019 Aug 9;10(1):3593
HeLa-JW cells
40 µM
1-2 hours
To investigate the effect of formin inhibitor SMIFH2 on filopodia adhesion, results showed that SMIFH2 treatment caused filopodia to detach from the beads, significantly shortening the contact time and weakening the exerted force.
Nat Commun. 2019 Aug 9;10(1):3593
HeLa cells
40 µM
20 minutes
Inhibition of IFNγ-induced STAT1 phosphorylation
Angew Chem Int Ed Engl. 2022 Aug 8;61(32):e202205231
Tobacco BY-2 cells
0.5 µM, 2.5 µM, 5 µM, 7.5 µM, 10 µM
5 days
SMIFH2 inhibits cell proliferation and causes cell death
搜索
