SLF1081851 operates as a Spns2 inhibitor, impeding S1P release (IC50=1.93 μM). This compound holds significant importance in the development and immune system[1][2].
体内研究
SLF1081851, administered intraperitoneally at a dose of 20 mg/kg, 4 hours postdose, significantly suppresses circulating lymphocytes and plasma S1P levels, while also reproducing the genetic phenotype observed in Spns2 null mice[1].
体外研究
Sphingosine 1-phosphate (S1P) serves as a multifaceted signaling molecule, while Spns2 is responsible for preserving lymph S1P levels[1].
SLF1081851 (0-5 μM; 18-20 h) hinders S1P release, displaying an IC50 value of 1.93 μM in Hela cells[1].
SLF1081851 (0-30 μM; 20 min) dose-dependently inhibits both mSphK1 (recombinant mouse SphK) (at 10 μM) and mSphK2 (at 5 μM), indicating at least 15-fold selectivity[1].
SLF1081851 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Human aortic smooth muscle cells (HAoSMCs)
3 µM
11 days
SLF1081851 significantly reduced HAoSMC calcification under calcifying conditions.
Pflugers Arch. 2025 Jun;477(6):815-826
HeLa cells
1.93 µM (IC50)
18-20 hours
To evaluate the inhibitory effect of SLF1081851 on S1P release, showing an IC50 of 1.93 μM.
J Med Chem. 2022 Jun 9;65(11):7656-7681
Human aortic smooth muscle cells (HAoSMCs)
3 µM
48 hours
SLF1081851 significantly suppressed calcification medium-induced CBFA1 and ALPL mRNA expression in HAoSMCs and reduced HAoSMC calcification.
Pflugers Arch. 2025 Jun;477(6):815-826
SLF1081851 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
C57BL/6j strain
Intraperitoneal injection
10 mg/kg
Single dose
To evaluate the effect of SLF1081851 on lymphopenia and plasma S1P levels in mice.
J Med Chem. 2023 Apr 27;66(8):5873-5891
SLF1081851 实验方案
计算器
存储液制备
1mg
5mg
10mg
1 mM
5 mM
10 mM
2.91mL
0.58mL
0.29mL
14.56mL
2.91mL
1.46mL
29.11mL
5.82mL
2.91mL
SLF1081851 技术信息
CAS号
2763730-97-6
分子式
C21H33N3O
分子量
343.51
SMILES Code
NCCCC1=NC(C2=CC=C(CCCCCCCCCC)C=C2)=NO1
MDL No.
MFCD34676462
别名
运输
蓝冰
存储条件
In solvent-20°C:3-6个月-80°C:12个月
Pure formKeep in dark place, inert atmosphere, 2-8°C
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