CD27 antigen, also known as CD27L receptor, T-cell activation antigen CD27, T14, S152, Tp55, TNFRSF7 and Tumor necrosis factor receptor for superfamily member 7，belongs to the TNF-receptor superfamily. CD27 is a single-pass type I membrane protein and exists as a homodimer form, containing three TNFR-Cys repeats. CD27 transduces signals that lead to the activation of NF-KappaB and MAPK8/JNK. CD27 is involved in regulating B-cell activation and immunoglobulin synthesis, binding to the ligand CD70. TRAF2 and TRAF5 have been shown to mediate the signaling process of CD27. CD27-binding protein (SIVA), which is a proapoptotic protein, can bind to CD27 and is thought to play a key role in the apoptosis. CD27 is required for generation and long-term maintenance of T cell immunity.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
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Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
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