货号:A2900869
同义名:
重组大鼠TNF alpha蛋白(E.coli, tag free)
/ Recombinant Rat TNF alpha; Tumor Necrosis Factor
Recombinant Rat TNF alpha由大肠杆菌表达系统生产,目标基因编码Leu80-Leu235。
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描述 | 产品名称:Recombinant Rat TNF alpha理论分子量:17.4 KDa蛋白种属:Rat表达系统:E.coli蛋白表达区间:Leu80-Leu235标签:Tag free剂型:干粉纯度:≥95% as determined by reducing SDS-PAGE. 储存条件:冻干蛋白应储存在≤-20°C,收到后可稳定保存一年。 溶解后的蛋白溶液可以在2-8°C下保存2-7天。如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。 分装后的蛋白在≤-20°C 下可稳定保存 3 个月。产品背景:Tumor necrosis factor alpha (TNF-alpha, TNFSF2) is the prototypic ligand of the TNF superfamily. Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF exists as a homotrimer and interacts with SPPL2B. TNF is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. TNF is a key cytokine in the development of several inflammatory disorders. It contributes to the development of type 2 diabetes throught its effects on insulin resistance and fatty acid metabolism. |
CAS号 | N/A |
分子量 | 17.4 KDa |
SMILES Code | NONE |
别名 | 重组大鼠TNF alpha蛋白(E.coli, tag free) ;Recombinant Rat TNF alpha; Tumor Necrosis Factor; Cachectin; TNF-Alpha; Tumor Necrosis Factor Ligand Superfamily Member 2; TNF-a; Tumor Necrosis Factor; Membrane Form; Tumor Necrosis Factor; Soluble Form; Tnf; Tnfa; Tnfsf2 |
运输 | 蓝冰 |
存储条件 |
In solvent -20°C: 3-6个月 -80°C: 12个月 Pure form Sealed in dry, store in freezer, under -20°C |