Recombinant Rat SCF (C-6His, E.coli)是大鼠来源的重组蛋白，在大肠杆菌中表达，带有C端6×His标签。该蛋白为干细胞因子（SCF），是受体型酪氨酸激酶KIT的配体，参与调控细胞存活与增殖、造血、干细胞维持、配子发生、肥大细胞发育与迁移、黑色素生成等过程，并通过激活PIK3R1、AKT1、GRB2、RAS、RAF1、MAPK及STAT家族成员等信号通路发挥作用。
Rat SCF (C-6His, E.coli)/大鼠SCF蛋白(C-6His, E.coli) 化学结构 CAS号：N/A
Rat SCF (C-6His, E.coli)/大鼠SCF蛋白(C-6His, E.coli) 3D分子结构 CAS号：N/A
Rat SCF (C-6His, E.coli)/大鼠SCF蛋白(C-6His, E.coli) 质控信息
Rat SCF (C-6His, E.coli)/大鼠SCF蛋白(C-6His, E.coli) 生物活性
描述
Stem cell factor (SCF), is the ligand for the receptor-type protein-tyrosine kinase KIT. It plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. It also promotes phosphorylation of PIK3R1, which is the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Rat SCF (C-6His, E.coli)/大鼠SCF蛋白(C-6His, E.coli) 技术信息
搜索
