货号:A2925117
同义名:
重组大鼠IgG2a Fc蛋白(Tag free, HEK293)
/ Recombinant Rat IgG2a Fc (HEK293); Ig gamma-2A chain C region
Recombinant Rat IgG2a Fc (HEK293)是来自大鼠的重组蛋白,在HEK293人源细胞中表达,为无标签蛋白。该蛋白为免疫球蛋白G(IgG)的Fc片段,属于IgG2a亚型,由两条相同的重链和两条相同的轻链组成Y形结构,其重链恒定区与IgG1和IgG2b有64 - 78%序列同源性,能有效与FcγR相互作用。


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| 描述 | Immunoglobulin G (IgG) is a type of antibody composed of four peptide chains—two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers.There are four IgG subclasses (IgG1, 2, 3, and 4) in humans, named in order of their abundance in serum. The IgG2a isotype was able to interact very efficiently with FcgammaR, and share approximately 64-78% amino acid sequence identity with IgG1 and IgG2b in the heavy chain constant region domains, CH1, CH2 and CH3, which cause the diffrence in the interaction with complement, NK and mast cells, induction or inhibition by specific cytokines, and ability to cross the placenta. |
| 蛋白种属 | Rat |
| 表达系统 | Human Cells |
| 标签 | Tag free |
| 蛋白表达区间 | Val98-Lys322 |
| 蛋白编号 | P20760 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 干粉 |
| 剂型描述 | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, pH 8.5. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。 分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 35.2 KDa |
| SMILES Code | NONE |
| 别名 | 重组大鼠IgG2a Fc蛋白(Tag free, HEK293) ;Recombinant Rat IgG2a Fc (HEK293); Ig gamma-2A chain C region; Igg-2a |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C: 3-6个月 -80°C: 12个月 Pure form Sealed in dry, store in freezer, under -20°C |
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