Recombinant Rat GM-CSF (C-6His, HEK293)是大鼠来源的重组蛋白，在HEK293人源细胞中表达，带有C端6×His标签。该蛋白即粒细胞-巨噬细胞集落刺激因子（GM-CSF），能够支持粒细胞-巨噬细胞祖细胞的体外克隆形成，也可促进红系、巨核细胞及嗜酸性粒细胞祖细胞生长，并对成熟造血细胞、单核/巨噬细胞、嗜酸性粒细胞及非造血细胞如内皮细胞具有调节作用，还能刺激某些肿瘤细胞系的增殖。
Rat GM-CSF (C-6His, HEK293)/大鼠GM-CSF蛋白(C-6His, HEK293) 化学结构 CAS号：N/A
Rat GM-CSF (C-6His, HEK293)/大鼠GM-CSF蛋白(C-6His, HEK293) 3D分子结构 CAS号：N/A
Rat GM-CSF (C-6His, HEK293)/大鼠GM-CSF蛋白(C-6His, HEK293) 质控信息
Rat GM-CSF (C-6His, HEK293)/大鼠GM-CSF蛋白(C-6His, HEK293) 生物活性
描述
Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factorthat can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by anumber of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cellsand fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophageprogenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. Onmature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on nonhematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF canalso stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma andadenocarcinoma cell lines.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Rat GM-CSF (C-6His, HEK293)/大鼠GM-CSF蛋白(C-6His, HEK293) 技术信息
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