RS-1 increases the DNA binding activity of RAD51. It can also act as an enhancer of homology-directed repair and CRISPR/Cas9 mediated knock-in efficiency.
RAD51 filament formation is a key step in homologous recombination repair. RS-1 is a small molecule that enhances RAD51 binding and filament stability. RS-1 at a concentration of 0, 1–25μM stimulated strand assimilation activity of RAD51. RS-1 at 7.5μM promoted resistance of human cells to cross-linking chemotherapy[3]. Treatment of embryos with 7.5μM of RS-1 resulted in a significantly higher knock-in rate compared with the control group (26.1% vs. 4.4%). RS-1 at a dose of 7.5 and 15 μM also improved TALEN- and Cas9-mediated knock-in efficiency in rabbit embryos[4].
RS-1 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Porcine embryo cells
7.5 µM and 15 µM
20 hours and 144 hours
To evaluate the effects of RS-1 on porcine embryo development in vitro. Results showed that transient exposure to 7.5 μM RS-1 did not affect embryo development, while 15 μM RS-1 reduced cleavage rates. Long-term exposure to 7.5 μM RS-1 decreased blastocyst formation rates.
Reprod Toxicol. 2021 Oct;105:44-52
Mouse SCNT embryos
10 µM
22 hours
RS-1 treatment significantly improved the blastocyst formation rate and enhanced embryo morphology and development.
Cell Prolif. 2021 Jul;54(7):e13059
Normal neonatal human dermal fibroblasts
1–7.5 µM
24 hours
Assess RS-1 effect on cisplatin resistance, RS-1 promotes dose-dependent resistance
Proc Natl Acad Sci U S A. 2008 Oct 14;105(41):15848-53
Tobacco leaf
25 µM
30 minutes
To assess the effect of RS-1 on tobacco leaves for subsequent Agrobacterium-mediated transformation experiments
Front Genome Ed. 2020 Nov 25;2:604289
Rice callus
25 µM
30 minutes
To evaluate the cytotoxicity of RS-1 on rice callus, showing no significant effect on growth at 25 µM RS-1
Front Genome Ed. 2020 Nov 25;2:604289
HEK293T cells
10 µM
42 hours
Stimulation of RAD51 significantly increased the knockin efficiency of the mito-Cas9 system
Innovation (Camb). 2022 Sep 27;3(6):100329
MRC-5 normal primary human fibroblasts
60 µM
6 hours
RS-1 treatment did not significantly induce RAD51 nuclear protein foci formation
Cancer Res. 2014 Jul 1;74(13):3546-55
PC3 prostate cancer cells
60 µM
6 hours
RS-1 treatment induced the formation of RAD51 nuclear protein foci, accompanied by cell death
Cancer Res. 2014 Jul 1;74(13):3546-55
RS-1 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
SCNT cloned embryos
Added to in vitro culture medium
10 µM
Single treatment, lasting 22 hours
RS-1 treatment significantly improved the developmental efficiency of SCNT cloned embryos and the birth rate of cloned mice.
Cell Prolif. 2021 Jul;54(7):e13059
Athymic nude mice
PC3 xenograft tumor model
Intraperitoneal injection
110 mg/kg
Once daily for 5 days
RS-1 treatment significantly inhibited tumor growth, with 43% of tumors completely disappearing
Cancer Res. 2014 Jul 1;74(13):3546-55
Rice (Oryza sativa L. cv. Nipponbare)
Rice callus
Medium treatment
25 µM
30 minutes treatment, followed by culture in medium containing 25 µM RS-1 for 2 weeks
To evaluate the effect of RS-1 on CRISPR/Cas9-mediated gene targeting frequency, showing a slight but consistent improvement in gene targeting frequency with RS-1 treatment
Front Genome Ed. 2020 Nov 25;2:604289
Pig
In vitro-produced embryo transfer model
In vitro culture
7.5 μM
20 hours
To evaluate the effects of RS-1 on porcine embryo development in vivo. Results showed that transient exposure to 7.5 μM RS-1 was compatible with in vivo development, successfully producing healthy piglets.
搜索
