Puromycin (2HCl), an aminonucleoside antibiotic, inhibits protein synthesis. Puromycin (2HCl), an analog of the 3' end of aminoacyl-tRNA, causes premature termination of translation by being linked non-specifically to growing polypeptide chains. Puromycin acting as a non-inhibitor at very low concentration (e.g. 0.04 microM) can bond only to full-length protein at the C-terminus. Puromycin and its derivatives at concentrations lower than those able to compete effectively with aminoacyl-tRNA can bond specifically to full-length protein at a stop codon[3]. Puromycin is a peptidyl nucleoside endowed with significant antibiotic and anticancer properties, but also with an unfortunate nephrotoxic character that has hampered its use as a chemotherapeutic agent[4]. Moreover, puromycin is a damaging agent known to induce apoptosis in mammalian cells. Puromycin-induced apoptosis in S. cerevisiae[5]. Carbocyclic puromycin was equal to puromycin when evaluated for cytotoxicity using P-388 mouse lymphoid leukemia cells in culture[6].
Puromycin 2HCl/嘌呤霉素二盐酸盐 细胞实验
Cell Line
Concentration
Treated Time
Description
References
CD4+ T cells
5 μg/mL
Inhibited the proliferation of CD4+ T cells and reduced their pathogenicity
Nat Commun. 2022 Oct 4;13(1):5866.
Mouse primary myotubes
10 μg/mL
10 min
To measure protein synthesis levels, results showed increased puromycin incorporation, indicating enhanced protein synthesis.
Skelet Muscle. 2019 Oct 31;9(1):26.
E14 mouse embryonic stem cells
1 μg/ml
48 h
To select clones transduced with Nm-gRNA
Cell Death Discov. 2023 Feb 13;9(1):61.
FaDu cells
2 μg/ml
1 week
To screen stably silenced and overexpressed IGF2BP2 HNSCC cells
J Exp Clin Cancer Res. 2022 Jan 3;41(1):6.
SCC15 cells
2 μg/ml
1 week
To screen stably silenced and overexpressed IGF2BP2 HNSCC cells
J Exp Clin Cancer Res. 2022 Jan 3;41(1):6.
Puromycin 2HCl/嘌呤霉素二盐酸盐 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
Experimental autoimmune uveitis (EAU) model
Added to the culture medium
5 μg/mL
Used to select transduced cells and inhibit the proliferation of CD4+ T cells
Nat Commun. 2022 Oct 4;13(1):5866.
Mice
SCID mice
Intraperitoneal injection
0.040 μM
Single injection, lasting 30 minutes
To measure muscle protein synthesis levels, results showed increased puromycin incorporation, indicating enhanced protein synthesis.
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