Propylthiouracil, known as 6-n-Propylthiouracil and classified as a thioamide antithyroid drug, acts as an inhibitor of thyroperoxidase and DIO1, suitable for use in research related to Graves' disease and hyperthyroidism [1].
体内研究
By feeding adult C57BL/6J and wild-derived WSB/EiJ male mice an iodine-deficient diet with 0.15% Propylthiouracil supplementation, Propylthiouracil triggers hypothyroidism [1].
体外研究
In U-937 cells, Propylthiouracil (5.5-330 μg/mL; 24 h) leads to dose-dependent growth inhibition and cytotoxic effects [2].
Propylthiouracil/丙硫氧嘧啶 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Mussel larvae
1 mM, 100 µM, 10 µM
24 hours
Investigate the effect of PTU on larval metamorphosis, results showed all concentrations of PTU significantly inhibited larval metamorphosis, with lower concentrations having a more pronounced effect
Sci Rep. 2021 Sep 29;11(1):19288.
Rat thyroid FRTL-5 cells
5 mM
24 to 72 hours
Radioiodine uptake studies showed that PTU increased 125I accumulation in FRTL-5 cells, but this effect was weaker than that produced by TSH.
Thyroid. 2012 Aug;22(8):844-52.
Rat thyroid FRTL-5 cells
5 mM
48 hours
RT-PCR and real-time PCR analysis revealed that PTU-induced NIS mRNA levels were comparable to those elicited by thyroid-stimulating hormone (TSH).
Thyroid. 2012 Aug;22(8):844-52.
Propylthiouracil/丙硫氧嘧啶 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Sprague-Dawley rats
Non-obese non-alcoholic fatty liver disease (NO-NAFLD) model
Dietary administration
0.2%
4 weeks
To evaluate the relevance of the HFD+PTU-induced rat model in mimicking the histopathology and pathophysiology of NO-NAFLD. Results showed that hepatic steatosis in the HFD+PTU model at week 4 was comparable to that of the HFD model at week 8, and the HFD+PTU model exhibited higher levels of carnitines, phospholipids, and triglycerides, supporting its suitability as a research tool for NO-NAFLD.
Int J Mol Sci. 2024 Oct 7;25(19):10764
Mussel juveniles
Mussel juveniles
Water exposure
0.01, 0.1, 1μM
4 weeks
Investigate the effect of PTU on juvenile growth, results showed PTU had no significant effect on juvenile growth
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