To evaluate the protective effect of OBB on LPS-induced barrier dysfunction in Caco-2 cells. Results showed that OBB improved TEER values, reduced FITC-dextran flux, and upregulated the expression of tight junction proteins ZO-1, occludin, and E-cadherin.
Front Pharmacol. 2025 Jan 7;15:1496874
RAW 264.7 macrophages
0.1, 0.2, 0.4 µM
24 hours
To evaluate the effect of OBB on LPS-induced secretion of inflammatory cytokines TNF-α, IL-6, and IL-18. Results showed that OBB significantly suppressed the secretion of these cytokines, and 0.4 μM OBB had comparable effects to 5 μM BBR.
Drug Des Devel Ther. 2022 Dec 23;16:4365-4383
RAW 264.7 macrophages
2.5, 5, 10, 20, 40, 80 µM
24 hours
To evaluate the cytotoxic effect of OBB and BBR on RAW 264.7 macrophages. Results showed that OBB exhibited no significant inhibition on cell growth at concentrations ranging from 2.5 to 80 μM, while BBR showed toxicity at 80 μM.
Drug Des Devel Ther. 2022 Dec 23;16:4365-4383
Huh7
1, 5, 10, 50, 100 µM
48 hours
OBB significantly inhibited USP7 expression in a dose-dependent manner.
Hepatol Commun. 2024 Mar 29;8(4):e0405
Hep3B
1, 5, 10, 50, 100 µM
48 hours
OBB significantly inhibited USP7 expression in a dose-dependent manner.
Hepatol Commun. 2024 Mar 29;8(4):e0405
Oxyberberine/氧化小檗碱 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Kunming mice
Acetic acid-induced vascular permeability mice model
Oral
1, 2, 4 mg/kg
For 7 consecutive days
To evaluate the effect of OBB on acetic acid-induced vascular permeability. Results showed that OBB significantly suppressed vascular permeability in a dose-dependent manner, and 2 mg/kg OBB was as effective as 20 mg/kg BBR.
Drug Des Devel Ther. 2022 Dec 23;16:4365-4383
Sprague Dawley rats
Hyperuricemia model
Oral
50 mg/kg
Single dose
To evaluate the pharmacokinetic properties of OBB-HP-β-CD SDDS, results showed its AUC0-t and Cmax were significantly higher than OBB raw material
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