There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
N-Ethylmaleimide (NEM), a reagent that alkylates free sulfhydryl groups, is a cysteine protease inhibitor[2]. N-ethylmaleimide (NEM) was shown to be a highly effective inhibitor of the following coupled mitochondrial processes: oxidative phosphorylation, ATP-32Pi exchange, Pi-induced light scattering and configurational changes, State III respiration, valinomycin-induced translocation of potassium with Pi as the anion, and calcium accumulation in presence of Pi. Also NEM, even et levels 2-3 times greater than those required to inhibit oxidative phosphorylation in intact mitochondria, did not inhibit coupled processes in submitochondrial particles[3]. When D. salina thylakoid membranes are treated with N-ethylmaleimide, both photophosphorylation and the inducible CF1 ATPase activity are partially (approx. 60%) inhibited. Treatment of the purified, latent, D. salina CF1 with low concentrations of N-ethylmaleimide also results in the partial (approx. 60%) inhibition of the inducible ATPase activity (IC50 approximately 50 mM). N-ethylmaleimide-induced inhibition of the ATPase activity of either membrane-bound or solubilized CF1 is partially reversed by either prolonged incubation at low concentrations of N-ethylmaleimide or short incubation times at high concentrations of N-ethylmaleimide[4].
*该产品中的马来酰亚胺基团在碱性水溶液中会发生水解形成开环的马来酸酰胺衍生物，会影响生物学活性，例如无法和巯基形成共价硫醚键，影响和生物大分子的连接，建议现配现用，避免在水溶液中长期保存，可在中性或弱酸性水溶液中低温保存，反应时控制弱酸环境[5][6]。
N-Ethylmaleimide/N-乙基马来酰亚胺 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Xenopus laevis oocytes
1 mM
10 minutes
To investigate the inhibitory effect of NEM on hCAT-2A, it was found that NEM inhibits hCAT-2A activity by modifying Cys-33 and Cys-273.
J Biol Chem. 2013 Oct 18;288(42):30411-30419.
CA1 pyramidal cells
250-300 µM
10-15 minutes
To test the effect of NEM on G-protein-mediated DSI (depolarization-induced suppression of inhibition). NEM was found to block DSI and enhance GABA release.
J Neurosci. 1997 Feb 1;17(3):941-50.
Immature cortical neurons
100 µM
15 minutes
To investigate the effect of NEM on KCC2 activity in neurons, results showed that NEM significantly reduced intracellular Cl⁻ concentration.
J Biol Chem. 2017 Dec 29;292(52):21253-21263.
HEK293 cells
100 µM
15 minutes
To investigate the effect of NEM on KCC2 function, results showed that NEM significantly increased KCC2 transporter activity.
J Biol Chem. 2017 Dec 29;292(52):21253-21263.
N-Ethylmaleimide/N-乙基马来酰亚胺 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Squid
Squid giant presynaptic terminal
Microinjection
0.05–2.5 mM
Action potentials elicited every 1 s
Inhibition of neurotransmitter release by photolysis of cNSF3 peptide, revealing the critical role of NSF in synaptic vesicle trafficking
Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):347-52
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