V-set and immunoglobulin domain containing 4 (VSIG4) is a type I transmembrane glycoprotein that is a B7 family-related protein and an Ig superfamily member. Mouse VSIG4 is synthesized as a 280 amino acid (aa) precursor that contains a signal sequence, an IgV-type immunological domain (aa 36-115),one potential N-linked glycosylation site, and a single transmembrane domain. The IgV domain of mouse VSIG4 shares 86% and 80% aa sequence identity with the IgV domains of rat and human VSIG4, respectively. VSIG4 functions as a negative regulator of mouse as well as human T cell activation, and may be involved in the maintenance of peripheral T cell tolerance and/or unresponsiveness. VSIG4 acts as a macrophage complement receptor by binding complement fragments C3b and iC3b. VSIG4 binding to C3b inhibits complement activation through the alternative pathway, making it a potent suppressor of established inflammation.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
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Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
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