货号:A2900696
同义名:
重组小鼠IBSP蛋白(HEK293, C-6His)
/ Recombinant Mouse IBSP (C-6His)
Recombinant Mouse IBSP (C-6His)由哺乳动物表达系统生产,目标基因编码Phe17-Gln324,并在C末端带有6His标签。
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描述 | 产品名称:Recombinant Mouse IBSP (C-6His)理论分子量:35.1 KDa蛋白种属:Mouse表达系统:Human Cells蛋白表达区间:Phe17-Gln324标签:C-6His剂型:干粉纯度:≥95% as determined by reducing SDS-PAGE.储存条件:冻干蛋白应储存在≤-20°C,收到后可稳定保存一年。 溶解后的蛋白溶液可以在2-8°C下保存2-7天。如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。 分装后的蛋白在≤-20°C 下可稳定保存 3 个月。产品背景:Bone sialoprotein 2(IBSP) is a monomeric non‑collagenous member of the SIBLING family of extracellular matrix proteins. It is principally associated with the early stages of bone mineralization. Mouse IBSP is synthesized as a 324 amino acid (aa) precursor that contains a 16 aa signal sequence and a 308 aa mature region. The mature segment is divided into a basic N‑terminus (aa 17 ‑ 62), a central region (aa 63 ‑ 233), and an acidic C‑terminus (aa 234 ‑ 317). IBSP is highly glycosylated, sulfated and phosphorylated. Phosphorylation promotes HAp nucleation, while carbohydrate may regulate cell adhesion. IBSP binds tightly to hydroxyapatite, appears to form an integral part of the mineralized matrix. It is probably important to cell-matrix interaction and promotes Arg-Gly-Asp-dependent cell attachment. |
CAS号 | N/A |
分子量 | 35.1 KDa |
SMILES Code | NONE |
别名 | 重组小鼠IBSP蛋白(HEK293, C-6His) ;Recombinant Mouse IBSP (C-6His) |
运输 | 蓝冰 |
存储条件 |
In solvent -20°C:3-6个月-80°C:12个月 Pure form Sealed in dry, store in freezer, under -20°C |