There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Human Umbilical Vein Endothelial Cells (HUVECs) are exposed to various concentrations of Methylthiouracil (MTU), ranging from 0 to 20 μM, for six hours following an initial treatment with LPS (100 ng/mL) for four hours. MTU effectively reduces LPS-induced hyperpermeability in these endothelial cells, showing maximal efficacy at concentrations exceeding 5 μM. Additionally, subsequent treatment with MTU at 10 or 20 μM suppresses the formation of LPS-induced paracellular gaps while promoting the development of dense F-actin rings. To evaluate MTU's safety, cellular viability assays are conducted on HUVECs treated with MTU for 24 hours, confirming that MTU up to 20 μM does not compromise cell viability[1].
体内研究
Methylthiouracil is also utilized in animal models to study cardiovascular and cerebrovascular diseases. Specifically, MTU treatment significantly reduces the peritoneal leakage of dye triggered by LPS. Given that the average circulating blood volume in mice is 72 mL/kg and the average weight of the mice used in this study is 27 g, with an estimated blood volume of 2 mL, the administration of MTU at doses of 142 or 284 μg/kg achieves a maximum concentration of 10 or 20 μM in the peripheral blood[1].
体外研究
Human Umbilical Vein Endothelial Cells (HUVECs) are exposed to various concentrations of Methylthiouracil (MTU), ranging from 0 to 20 μM, for six hours following an initial treatment with LPS (100 ng/mL) for four hours. MTU effectively reduces LPS-induced hyperpermeability in these endothelial cells, showing maximal efficacy at concentrations exceeding 5 μM. Additionally, subsequent treatment with MTU at 10 or 20 μM suppresses the formation of LPS-induced paracellular gaps while promoting the development of dense F-actin rings. To evaluate MTU's safety, cellular viability assays are conducted on HUVECs treated with MTU for 24 hours, confirming that MTU up to 20 μM does not compromise cell viability[1].
Methylthiouracil/甲基硫脲嘧啶 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Human umbilical vein endothelial cells (HUVECs)
0-20 μM
6 hrs
MTU suppressed PolyP-mediated vascular barrier permeability
J Cell Mol Med. 2016 Dec;20(12):2333-2340
B16F10 cells
266 μM（IC50）
48 hours
Evaluate the effect of Methylthiouracil on melanin content, showing no cytotoxicity at 20 μM but no significant reduction in melanin content.
Int J Mol Sci. 2015 Dec 2;16(12):28534-48
rat bone marrow stromal cells
10, 20, 40, 80, 160, 320 mmol/l
48 hours
To investigate the effect of MTU on BMSC apoptosis. Results showed that MTU promoted BMSC apoptosis, and the apoptosis rate increased with increasing concentration.
Exp Ther Med. 2014 Jun;7(6):1738-1744
Methylthiouracil/甲基硫脲嘧啶 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Wistar-Kyoto rats
Atherosclerosis model induced by cholesterol-rich diet plus methylthiouracil
Gavage
80 mg/kg/day
Once daily for five months
To investigate the role of methylthiouracil in a cholesterol-rich diet-induced atherosclerosis model. Results showed that cholesterol-rich diet plus methylthiouracil led to lipid deposition, VSMC proliferation, and migration toward intima in aortic tissues, which were significantly alleviated by rosiglitazone treatment.
Cardiovasc Diabetol. 2011 Jan 26;10:10
C57BL/6 mice
PolyP-induced vascular inflammatory model
Intravenous injection
28-284 μg/kg
Single or twice administration (at 12 hrs and 50 hrs post-PolyP)
MTU suppressed PolyP-mediated vascular permeability and leucocyte migration, increased survival rate, and reduced hepatic and renal injury markers
J Cell Mol Med. 2016 Dec;20(12):2333-2340
Rat
Congenital hypothyroidism model
Oral
0.1%（drinking water）
From gestation day 16 until sacrifice postnatally
To study the effect of thyroid hormone on microglial development, results showed hypothyroidism led to reduced microglial cell numbers and processes.
J Neurosci. 2001 Mar 15;21(6):2028-38
Rats
Normal rats
Subcutaneous injection
0.02g
Daily for seven days
To assess the inhibitory effect of methylthiouracil on thyroid activity. Results showed that follicular cells possessed more numerous and larger microvilli (up to 2.0 μm), dilated cisternae of endoplasmic reticulum, elongated mitochondria (up to 2.3 μm), and more oval or indented nuclei.
J Anat. 1982 Sep;135(Pt 2):407-12
Rats
Intact and castrate rats
Drinking water
20mg/day
Continuous administration
Methylthiouracil accelerates and increases the induction of cervico-vaginal sarcomas and epithelial tumours in both intact and castrate rats.
Br J Cancer. 1970 Sep;24(3):510-27
Rats
Castrate rats
Drinking water
20mg/day
Continuous administration
Methylthiouracil lowers the threshold for basal celled vulval neoplasms and promotes the formation of basal celled vulval tumours.
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