L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate is a vitamin derivative that stimulates collagen formation and expression[1].L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate can be used as a medium supplement for osteogenic differentiation of human adipose stem cells (hASCs). During osteogenic differentiation, L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate increased alkaline phosphatase (ALP) activity and runx2A expression in human adipose stem cells[2][3].The addition of L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate significantly stimulated cell growth while the addition of L-ascorbic acid (Asc) only achieved weak growth stimulation at concentrations of 0.1-1.5 mM with medium changes every 2 to 3 days for 2-3 weeks[1].Effective osteogenic differentiation of human adipose stem cells (hASCs) requires L-Ascorbic acid 2-phosphate sesquimagnesium salt hydrate (50 µM-250 µM), and higher concentrations of AsA2-P increase runx2 expression and ALP activity. 150 µM AsA2-P and 10 nM concentration of dexamethasone and 250 µM AsA2-P or 5 nM concentration of dexamethasone resulted in the highest proliferation, ALP activity and runx2 expression[3].
Used for differentiation of hESCs into hepatoblasts and ultimately hepatocyte-like cells (HLCs). Results showed that HLCs possessed typical hepatocyte morphology and characteristics of mature hepatocytes, such as metabolic-related gene expression, albumin secretion, fat accumulation, glycogen storage, and inducible cytochrome P450 activity.
Cell Death Dis. 2019 Oct 10;10(10):763
porcine skin
13 mM
12 hours
To study the permeability of MAP-1 and MAP-2 in porcine skin. Results showed that CO2 laser pretreatment significantly enhanced the permeability of MAP-1 and MAP-2.
Molecules. 2016 Nov 16;21(11):1547
porcine cumulus-oocyte complexes (COCs)
250 μM
44 hours
significantly enhanced the nuclear maturation (as indicated by higher rate of first polar body extrusion and increased Bmp15 mRNA level), reduced level of reactive oxygen species, and promoted developmental potency
Sci Rep. 2018 Apr 17;8(1):6132
Umbilical cord blood hematopoietic stem and progenitor cells
150 μg/mL
From day 3 of differentiation
Promotes erythroid differentiation, increases the proportion of late basophilic erythroblasts, upregulates the expression of erythroid-related regulatory molecules GATA1, KLF1, ALAS2, and the globins HBG and HBB.
搜索
