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L-Ascorbic Acid/抗坏血酸 {[allProObj[0].p_purity_real_show]}

货号:A182821 同义名: L-抗坏血酸;维生素C / L-Ascorbate; Vitamin C

L-Ascorbic Acid是一种天然抗氧化剂,选择性抑制 Cav3.2 通道 (IC50=6.5 μM),并通过产生活性氧 (ROS) 选择性损伤癌细胞,显示出抗癌潜力。此外,它还是胶原生成的促进剂,在皮肤护理和癌症研究中具有广泛应用。

L-Ascorbic Acid/抗坏血酸 化学结构 CAS号:50-81-7
L-Ascorbic Acid/抗坏血酸 化学结构
CAS号:50-81-7
L-Ascorbic Acid/抗坏血酸 3D分子结构
CAS号:50-81-7
L-Ascorbic Acid/抗坏血酸 化学结构 CAS号:50-81-7
L-Ascorbic Acid/抗坏血酸 3D分子结构 CAS号:50-81-7
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L-Ascorbic Acid/抗坏血酸 纯度/质量文件 产品仅供科研

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L-Ascorbic Acid/抗坏血酸 生物活性

描述 L-Ascorbic Acid, also named as Vitamin C, is a water-soluble compound, widely existing in many vegetables and fruits. In vivo studies, L-ascorbic acid could promote the proliferation of human epithelial HaCaT cells [1] . Besides this, it was found that L-ascorbic acid could also suppress the leukemic cell colony in simultaneous cultures, even at the concentration of 0.1 mM [2] . In vivo studies, L-ascorbic acid has the ability of protecting lead toxicated rats against anemia [3] . In addition, it was observed that L-ascorbic acid contributed to the improvement of examined electrophysiological parameters in rats [4] .

L-Ascorbic Acid/抗坏血酸 细胞实验

Cell Line
Concentration Treated Time Description References
Human whole blood samples 6 µM 10 minutes To evaluate the effect of L-ascorbic acid on tissue-type plasminogen activator-mediated fibrinolysis. Results showed that L-ascorbic acid significantly enhanced tPA-mediated fibrinolysis. Int J Mol Sci. 2020 Jun 1;21(11):3972.
Rabbit Achilles tenocytes 50 μg/mL 14 days To evaluate the effect of L-ascorbic acid on extracellular matrix deposition by tenocytes. L-ascorbic acid significantly accelerated collagen I deposition, visible as early as day 3, with the collagen I matrix becoming denser over time. Fibronectin deposition was also increased. Int J Mol Sci. 2020 Jan 10;21(2):458.
Mouse hippocampal slice CA3-CA1 synapses 0.2 mM, 0.4 mM, 1 mM, 2 mM 15-20 minutes Evaluated AA effects on synaptic transmission, showing concentration-dependent inhibition of fEPSPs (57% inhibition at 0.2 mM, 77% at 2 mM) without altering synaptic excitability (no change in V50). Nutrients. 2022 Jan 30;14(3):613.
Human skin fibroblasts 100 µM 24 hours To evaluate the protective effects of L-ascorbic acid and rutin on UVB-irradiated human skin fibroblasts. Results showed that the combination of L-ascorbic acid and rutin significantly reduced UVB-induced oxidative stress and protein modifications, improving cell viability. Nutrients. 2020 Apr 13;12(4):1074.
HDF cells 1 mg/mL 48 hours Evaluation of the free radical scavenging activity of L-AA, which showed a scavenging activity of 82.00 ± 15.28%. Mar Drugs. 2023 Aug 30;21(9):479.
Human embryonic stem cells (hESCs)/induced pluripotent stem cells (iPSCs) 50 μg/mL Long-term culture Promote the generation and long-term expansion of long-term self-renewing neuroepithelial-like stem cells (lt-NES cells) by promoting collagen synthesis Stem Cell Res Ther. 2021 Jan 9;12(1):48.
Fibroblasts from a healthy donor and a type II SMA patient 400 µM To evaluate the effect of L-ascorbic acid on cell viability, results showed that L-AA protected cells against vanadate-induced cell death. BMC Med. 2013 Feb 14;11:38.
NG108-15 cells 0.1–1 mM 2 min To verify the inhibitory effect of ascorbic acid on NaHS-induced facilitation of T-type currents Br J Pharmacol. 2012 Jun;166(3):1058-68

L-Ascorbic Acid/抗坏血酸 动物实验

Species
Animal Model
Administration Dosage Frequency Description References
Hairless mice UVB-induced skin photoaging model Oral 100 mg/kg Once daily for 15 weeks Evaluation of the protective effects of L-AA against UVB-induced skin photoaging, showing significant inhibition of UVB-induced skin wrinkle formation and skin water loss. Mar Drugs. 2023 Aug 30;21(9):479.
New Zealand white rabbits Corneal endothelial damage model Topical application 284 mmol/L Three times a day for two days To investigate the protective effect of topical L-Ascorbic Acid on oxidative stress-induced corneal endothelial damage. Results showed that L-Ascorbic Acid significantly increased its concentration in the corneal stroma and improved corneal clarity. Cells. 2020 Apr 11;9(4):943
Mice Late-onset SMA Mice model Oral 40 mg/kg Once daily from postnatal days 1 to 30 To evaluate the therapeutic potential of L-ascorbic acid in SMA mice, results showed that combined treatment delayed disease progression, improved motor performance in adulthood, enhanced SMN levels and motor neuron numbers, reduced muscle atrophy, and decreased Bax levels in the spinal cord. BMC Med. 2013 Feb 14;11:38.

L-Ascorbic Acid/抗坏血酸 临床研究

NCT号 适应症或疾病 临床期 招募状态 预计完成时间 地点
NCT02553551 Chronic Cholecystitis ... 展开 >> Cholelithiasis 收起 << Not Applicable Unknown September 2017 Korea, Republic of ... 展开 >> Department of HBP Surgery, Seoul St. Mary's hospital Recruiting Seoul, Seocho-gu, Banopo-dong, Korea, Republic of, 137-701 Contact: Taeho Hong    +82-2-2258-6108    gshth@catholic.ac.kr 收起 <<
NCT01080352 Prostatic Neoplasms Phase 2 Completed - Denmark ... 展开 >> Departmen of Urology, Copenhagen University Hospital at Herlev Herlev, DK, Denmark, 2730 收起 <<
NCT02186158 Pneumonia With Hospitalized El... 展开 >>derly Patient 收起 << Phase 2 Completed - France ... 展开 >> CHU de Bordeaux, Hôpital Saint André Bordeaux, France, 33000 收起 <<

L-Ascorbic Acid/抗坏血酸 参考文献

[1]Lam PL, Kok SH, et al. Microencapsulation-protected l-ascorbic acid for the application of human epithelial HaCaT cell proliferation. J Microencapsul. 2014;31(8):754-8.

[2]Park CH, Amare M, et al. Growth suppression of human leukemic cells in vitro by L-ascorbic acid. Cancer Res. 1980;40(4):1062-5.

[3]Rudra PD, Chatterjee J, Chatterjee GC. Influence of lead administration on L-ascorbic acid metabolism in rats: effect of L-ascorbic acid supplementation. Int J Vitam Nutr Res. 1975;45(4):429-37.

[4]Kosik-Bogacka DI, Baranowska-Bosiacka I, et al. The effect of L-ascorbic acid and/or tocopherol supplementation on electrophysiological parameters of the colon of rats chronically exposed to lead. Med Sci Monit. 2011;17(1):BR16-26.

L-Ascorbic Acid/抗坏血酸 实验方案

计算器
存储液制备 1mg 5mg 10mg

1 mM

5 mM

10 mM

5.68mL

1.14mL

0.57mL

28.39mL

5.68mL

2.84mL

56.78mL

11.36mL

5.68mL

L-Ascorbic Acid/抗坏血酸 技术信息

CAS号50-81-7
分子式C6H8O6
分子量 176.12
SMILES Code O=C1O[C@H]([C@@H](O)CO)C(O)=C1O
MDL No. MFCD00064328
别名 L-抗坏血酸;维生素C ;L-Ascorbate; Vitamin C; Ascorbic acid, L-Ascorbic acid; NSC 218455; NSC 33832; Ascorbate
运输蓝冰
InChI Key CIWBSHSKHKDKBQ-JLAZNSOCSA-N
Pubchem ID 54670067
存储条件

In solvent -20°C: 3-6个月 -80°C: 12个月

Pure form Keep in dark place, sealed in dry, room temperature

溶解方案

DMSO: 105 mg/mL(596.18 mM),配合低频超声助溶,注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO

H2O: 100 mg/mL(567.79 mM)

配制的工作液建议现用现配,短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶
方案 一
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