Isochlorogenic acid C, a natural product isolated and purified from the herbs of Laggera alata, possesses potent hepatoprotective and anti-HBV, anti-HCV, and anti-HIV effects.
Isochlorogenic acid C/异绿原酸C 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HepG2/CH-Gal3 cells
750 µM
2 h
Monitored lysophagic flux, demonstrating reduced mCherry+/SEpHluorin-Gal3 puncta after LLOMe treatment, reversed by FBXO3 inhibition.
Antioxidants (Basel). 2019 Sep 8;8(9):381
HepG2/GFP-Gal3 cells
750 µM
2 h
Evaluated clearance of GFP-Gal3 puncta post-lysosomal damage induced by LLOMe, showing FBXO3 inhibition significantly impaired damaged lysosome clearance.
Antioxidants (Basel). 2019 Sep 8;8(9):381
HaCaT cells
5, 10, 20 μM
24 hours
Evaluate the protective effect of TCQA against UVB-induced oxidative stress, results showed TCQA significantly reduced ROS levels
Antioxidants (Basel). 2023 Oct 23;12(10):1899
HaCaT cells
5, 10, 20 μM
24 hours
Evaluate the effect of TCQA on the Nrf2–ARE pathway, results showed TCQA significantly increased Nrf2–ARE luciferase activity
Antioxidants (Basel). 2023 Oct 23;12(10):1899
RAW264.7 macrophages
1.56-25 μM
18 hours
To detect the cholesterol efflux-promoting ability of ICAC, results showed ICAC significantly promoted cholesterol efflux in a dose-dependent manner
Front Pharmacol. 2022 Jul 25;13:881078
Bone marrow-derived macrophages (BMMs)
1.56-50 μM
24 hours
To evaluate the cytotoxicity of ICAC on BMMs, results showed no significant cytotoxicity at concentrations below 50 μM
Front Pharmacol. 2022 Jul 25;13:881078
Human foreskin fibroblasts (HFF-1)
5, 10, 20 µM
24 hours
To investigate the protective effect of isochlorogenic acid C against blue light damage, results showed that isochlorogenic acid C at 5, 10, 20 µM significantly promoted COL-I secretion and inhibited MMP-1 secretion.
Molecules. 2023 Oct 28;28(21):7319
Isochlorogenic acid C/异绿原酸C 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Drosophila
Transgenic larval fat body
Oral
5 mM
2 h treatment, followed by transfer to normal food for 4-8 h
Validated the role of TBK1-FBXO3-TMEM192-TAX1BP1 axis in vivo lysophagy, showing impaired clearance of mCherry+/SEpHluorin-Gal3 puncta upon FBXO3 knockdown.
Antioxidants (Basel). 2019 Sep 8;8(9):381
C57BL/6 mice
High-fat diet-induced hyperlipidemia model
10 mg/kg/d and 20 mg/kg/d
Daily administration for 12 weeks
To evaluate the lipid-lowering effect of ICAC in HFD-induced hyperlipidemic mice, results showed ICAC significantly reduced lipid levels in serum and liver, and functioned by promoting the RCT pathway in liver and intestine
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