Isochlorogenic acid A is widely present in fruits, vegetables and herbal medicines, and is characterized by anti-inflammatory, hepatoprotective and antiviral properties[3]. The effects of oxidant stress, pulmonary edema and inflammation factors induced by LPS were inhibited by isochlorogenic acid A. Levels of lung active markers and inflammation factors induced by LPS were reversed by isochlorogenic acid A. Isochlorogenic acid A inhibited the cell apoptosis induced by LPS. The protein expressions of Nf-κB/NLRP3 signaling pathway induced by LPS were inhibited by isochlorogenic acid A[4]. Isochlorogenic acid A, also called 3,5-dicaffeoylquinic acid (3,5-diCQA), is a widespread phenolic compound in the plant. 3,5-diCQA upregulated intracellular melanin production in a time- and dose-dependent manner. Tyrosinase (TYR) activity was also increased after treatment with 3,5-diCQA in a dose-dependent manner. Expressions of TYR, TYR-related protein 1, TYR-related protein 2, and microphthalmia-associated transcription factor were upregulated in a dose-dependent manner after 48 h of treatment with 3,5-diCQA. Moreover, 3,5-diCQA increased the content of β-catenin in cell cytoplasm and nucleus by reducing the content of phosphorylated β-catenin (p-β-catenin)[5].
Isochlorogenic acid A/异绿原酸A 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HaCaT cells
10 μmol/L and 100 μmol/L
12 hours
To test the effect of isochlorogenic acid A on HaCaT cell death, results showed that IAB dose-dependently reduced HaCaT cell death.
Acta Pharm Sin B. 2022 Feb;12(2):723-734
HEK293 cells
50 μmol/L
To test the inhibitory effect of isochlorogenic acid A on TRPV3 currents, results showed that 50 μmol/L IAA significantly inhibited TRPV3 currents.
Acta Pharm Sin B. 2022 Feb;12(2):723-734
HaCaT cells
5, 10, 20 μM
24 hours
Evaluate the effect of TCQA on ROS levels, results showed TCQA concentration-dependently reduced ROS levels
Antioxidants (Basel). 2023 Oct 23;12(10):1899
HaCaT cells
5, 10, 20 μM
24 hours
Evaluate the effect of TCQA on the Nrf2–ARE pathway, results showed TCQA significantly increased Nrf2–ARE luciferase activity
Antioxidants (Basel). 2023 Oct 23;12(10):1899
HepaRG cells
1.25, 2.5, 5, 10, 25 μg/mL
12 hours
Evaluate the antioxidant activity of isochlorogenic acid A on H2O2-damaged HepaRG cells, results showed that isochlorogenic acid A significantly increased cell survival rate.
Food Chem X. 2024 Mar 16;22:101293
Isochlorogenic acid A/异绿原酸A 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
4T1 murine model
Intraperitoneal injection
10 mg/kg
Once daily for 14 days
Evaluate the inhibitory effect of ICGA-A on the growth of 4T1 TNBC tumors in vivo, results showed that ICGA-A significantly inhibited tumor growth.
Front Immunol. 2025 Mar 5;16:1529710
C57BL/6J mice
Dermatitis and chronic pruritus model
Topical administration
1.0 mmol/L
Once daily for 6 consecutive days
To test the effect of isochlorogenic acid A on mouse ear swelling and chronic pruritus, results showed that IAA and IAB significantly alleviated ear swelling and scratching behavior.
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