Isoacteoside is a natural compound isolated and purified from the herb of Pedicularis striata Pall. which exhibit significant inhibition of advanced glycation end product formation with IC50 values of 4.6-25.7 μM, compared with those of aminoguanidine (IC50=1,056 μM) and quercetin (IC50=28.4 μM) as positive controls, and exhibits anti-inflammatory effects through blocking TLR4 dimerization.
Isoacteoside/异类叶升麻苷 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Bone marrow-derived macrophages (BMDMs)
20, 40, 80 μM
24 hours
To evaluate the inhibitory effect of Isoacteoside on LPS-induced cytokine release, results showed that Isoacteoside significantly suppressed the secretion of TNF-α, IL-6, and IL-1β.
Br J Pharmacol. 2017 Sep;174(17):2880-2896
HEK293T cells
80 μM
24 hours
To evaluate the effect of Isoacteoside on TLR4 dimerization, results showed that Isoacteoside significantly inhibited LPS-induced TLR4 dimerization.
Br J Pharmacol. 2017 Sep;174(17):2880-2896
RAW264.7 macrophages
20, 40, 80 μM
24 hours
To evaluate the inhibitory effect of Isoacteoside on LPS-induced inflammatory responses, results showed that Isoacteoside significantly suppressed the expression of COX-2, iNOS, TNF-α, IL-6, and IL-1β.
Br J Pharmacol. 2017 Sep;174(17):2880-2896
SH-SY5Y cells
50 μg/mL
24 hours
Protected SH-SY5Y cells against Aβ1-42-induced neural damage and restored cell viability
Int J Mol Sci. 2017 Apr 24;18(4):895
Isoacteoside/异类叶升麻苷 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
BALB/c nude mice
HCC xenograft model
Intraperitoneal injection
30 mg/kg
Once daily for two weeks
The combination of Isoacteoside and sorafenib significantly inhibits tumor growth, showing better efficacy than sorafenib monotherapy.
Commun Biol. 2025 Feb 8;8(1):205
BALB/c mice
Xylene-induced ear oedema model, LPS-induced endotoxic shock model, and LPS-induced endotoxaemia-associated acute kidney injury (AKI) model
Single dose (ear oedema and endotoxic shock models); single dose and observed for 132 hours (endotoxic shock model); single dose and samples collected after 12 hours (AKI model)
To evaluate the anti-inflammatory effects of Isoacteoside in vivo, results showed that Isoacteoside significantly inhibited xylene-induced ear oedema, LPS-induced endotoxic death, and protected against LPS-induced acute kidney injury.
Br J Pharmacol. 2017 Sep;174(17):2880-2896
Sprague-Dawley rats
Aβ1-42-induced Alzheimer's disease model
Oral
2.5, 5.0 mg/kg
Once daily for 14 days
Ameliorated cognitive deficits induced by Aβ1-42, reduced amyloid deposition, and restored central cholinergic and monoaminergic function
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