Vinculin is a focal adhesion and cytoskeletal protein that distributed mainly at cell-cell junctions and cell-extracellular matrix (ECM) adhesion that belongs to the Vinculin/α-Catenin family. Vinculin is an Actin-binding protein and component of the Actin-Linking Functional module that senses and feels the mechanical properties of the extracellular environment. Vinculin is also a key factor that couples, transmits, transduces, and regulates mechanical force between the cytoskeleton and adhesion receptors. Vinculin generally forms two structural states, an open (active) and closed (inactive) state, which are controlled by conformational interaction(s) between the head and tail domains. Vinculin is involved in the mechano-chemical signal transmission of cells by binding to a variety of focal adhesion or cytoskeletal proteins, and plays important roles in cell adhesion, extension, motion, proliferation and survival.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human Vinculin/人Vinculin蛋白(E.coli, tag free) 技术信息
CAS号
N/A
分子量
117 KDa
SMILES Code
NONE
别名
重组人Vinculin蛋白(E.coli, tag free)
;Recombinant Human Vinculin; Vinculin; Metavinculin; VCL
运输
蓝冰
存储条件
In solvent-20°C:3-6个月-80°C:12个月
Pure formSealed in dry, store in freezer, under -20°C
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Tags:
Human Vinculin/人Vinculin蛋白(E.coli, tag free)
|Human Vinculin/人Vinculin蛋白(E.coli, tag free)
| 重组人Vinculin蛋白(E.coli, tag free)
| Recombinant Human Vinculin
| Vinculin
| Metavinculin
| VCL
| N/A
| Other Target Proteins
| Human Vinculin/人Vinculin蛋白(E.coli, tag free) 纯度/质量文件
| Human Vinculin/人Vinculin蛋白(E.coli, tag free) 生物活性
| Human Vinculin/人Vinculin蛋白(E.coli, tag free) 技术信息
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