货号:A2900878
同义名:
重组人VEGF165蛋白(HEK293, tag free)
/ Recombinant Human VEGF165; Vascular Endothelial Growth Factor Isoform 165
Recombinant Human VEGF165是人源血管内皮生长因子165,在人源细胞中表达,为无标签蛋白。该蛋白是VEGF-A最丰富的亚型,为含两条糖基化165氨基酸多肽链的二硫键连接同源二聚体,属于血小板衍生生长因子家族,是血管生成和血管发生的重要激活因子,主要通过与VEGFR1和VEGFR2结合发挥作用,其中VEGFR2介导其主要细胞应答。


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| 描述 | Human Vascular endothelial growth factor (VEGF), also known as VEGF-A and vascular permeability factor (VPF), belongs to the platelet-derived growth factor family of cysteine-knot growth factors. It is a potent activator in vasculogenesis and angiogenesis both physiologically and pathologically. VEGF-A has 8 differently spliced isoforms, of which VEGF165 is the most abundant one. VEGF165 is a disulfide-linked homodimer consisting of two glycosylated 165 amino acid polypeptide chains. VEGF stimulates the cellular response through binding to tyrosine kinase receptors VEGFR1 and VEGFR2 on the cell surface. It is widely accepted that VEGFR2 mediate almost all of the known cellular responses to VEGF while the function of VEGFR1 is less defined and is thought to modulate the VEGFR2 signaling. |
| 蛋白种属 | Human |
| 表达系统 | Human Cells |
| 标签 | Tag free |
| 蛋白表达区间 | Ala27-Arg191 |
| 蛋白编号 | P15692-4 |
| 内毒素 | < 0.01 EU/µg as determined by LAL test. |
| 剂型 | 干粉 |
| 剂型描述 | Lyophilized from a 0.2 μm filtered solution of 20mM Citrate, 8% Sucrose, 4% Mannitol, 0.05% Tween 80, pH4.0. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。 分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 19.1 KDa |
| SMILES Code | NONE |
| 别名 | 重组人VEGF165蛋白(HEK293, tag free) ;Recombinant Human VEGF165; Vascular Endothelial Growth Factor Isoform 165; VEGF165 |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C: 3-6个月 -80°C: 12个月 Pure form Sealed in dry, store in freezer, under -20°C |
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