货号:A2925789
同义名:
重组人UBE2H蛋白(N-GST, E.coli)
/ Recombinant Human UBE2H (N-GST, E.coli); Ubiquitin-Conjugating Enzyme E2 H
Recombinant Human UBE2H (N-GST, E.coli) 是人源泛素结合酶E2 H蛋白,在大肠杆菌中表达,带有N端GST标签。该蛋白属于E2泛素结合酶家族,参与泛素化过程,介导异常或短寿命蛋白的降解。研究表明其可在E3依赖下将泛素连接到组蛋白H2A,并与酵母DNA修复基因RAD6同源,受DNA损伤试剂诱导,与癌症恶病质及脓毒症诱导的肌肉蛋白水解调控相关。


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| 描述 | Ubiquitin-Conjugating Enzyme E2 H (UBE2H) belongs to the E2 Ubiquitin-Conjugating Enzyme family. The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. It has been shown to conjugate ubiquitin to histone H2A in an E3 dependent manner in vitro. UBE2H is the human homolog to the yeast DNA repair gene RAD6, which is induced by DNA damaging reagents. UBE2H has been associated with cancer-induced cachexia and with the regulation of sepsis-induced muscle proteolysis. |
| 蛋白种属 | Human |
| 表达系统 | E.coli |
| 标签 | N-GST |
| 蛋白表达区间 | Met1-Leu183 |
| 蛋白编号 | P62256 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 液体 |
| 剂型描述 | Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 2mM DTT, 10% Glycerol, pH 7.5. |
| 复溶 | 如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 47 KDa |
| SMILES Code | NONE |
| 别名 | 重组人UBE2H蛋白(N-GST, E.coli) ;Recombinant Human UBE2H (N-GST, E.coli); Ubiquitin-Conjugating Enzyme E2 H; UbcH2; Ubiquitin Carrier Protein H; Ubiquitin-Conjugating Enzyme E2-20K; Ubiquitin-Protein Ligase H; UBE2H |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C:3-6个月-80°C:12个月 |
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