货号:A2924456
同义名:
重组人Tie-1蛋白(C-6His, HEK293)
/ Recombinant Human Tie-1 (C-6His, HEK293); Tyrosine-Protein Kinase Receptor Tie-1
Recombinant Human Tie-1 (C-6His, HEK293) 是人源TIE-1蛋白,在HEK293细胞中表达,带有C端6×His标签。该蛋白是TIE受体酪氨酸激酶家族成员之一,与TIE-2/Tek共同在血管内皮及造血祖细胞中表达,参与血管生成、血管发生和造血过程。人源TIE-1前体蛋白含1124个氨基酸,包括信号肽、胞外域和胞内域,目前尚未发现其配体。


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| 描述 | TIE-1 (Tyrosine Kinase with Ig and EGF Homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily. These receptors are expressed on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-1 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18aa signal peptide, a 727 aa extracellular domain and a 354 aa cytoplasmic domain. so far, two ligands have been described for TIE-2 [angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2)], but no ligand was found for TIE-1. |
| 蛋白种属 | Human |
| 表达系统 | Human Cells |
| 标签 | C-6His |
| 蛋白表达区间 | Ala22-Gln760 |
| 蛋白编号 | P35590 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 干粉 |
| 剂型描述 | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 10% Sucrose, pH8.2. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。 分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 81 KDa |
| SMILES Code | NONE |
| 别名 | 重组人Tie-1蛋白(C-6His, HEK293) ;Recombinant Human Tie-1 (C-6His, HEK293); Tyrosine-Protein Kinase Receptor Tie-1; TIE1; TIE |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C: 3-6个月 -80°C: 12个月 Pure form Sealed in dry, store in freezer, under -20°C |
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