货号:A2925829
同义名:
重组人TIM蛋白(N-6His, E.coli)
/ Recombinant Human TIM (N-6His, E.coli); Triosephosphate Isomerase
Recombinant Human TIM (N-6His, E.coli) 是人源三磷酸异构酶(TIM/TPI),在大肠杆菌中表达,带有N端6×His标签。该蛋白催化二羟丙酮磷酸与D-甘油醛-3-磷酸之间的可逆转化,在糖酵解中起关键作用,对高效能量代谢至关重要。TPI缺乏会导致严重的溶血性贫血、神经肌肉功能障碍及感染易感性增加。


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| 描述 | Triose-phosphate isomerase, also named Triose-phosphate isomerase, TPI and TIM, is an enzyme that catalyzes the reversible interconversion of the triose phosphate isomers dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate. TPI has been found in nearly every organism searched for the enzyme, including animals such as mammals and insects as well as in fungi, plants, and bacteria. However, some bacteria that do not perform glycolysis, like ureaplasmas, lack TPI. TPI plays an important role in glycolysis and is essential for efficient energy production. TPI deficiency is an autosomal recessive disorder and the most severe clinical disorder of glycolysis. Triose phosphate isomerase deficiency is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection and characterized by chronic hemolytic anemia. |
| 蛋白种属 | Human |
| 表达系统 | E.coli |
| 标签 | N-6His |
| 蛋白表达区间 | Met1-Gln249 |
| 蛋白编号 | P60174 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 液体 |
| 剂型描述 | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 1mM DTT, 10% Glycerol, pH 8.0. |
| 复溶 | 如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 28.8 KDa |
| SMILES Code | NONE |
| 别名 | 重组人TIM蛋白(N-6His, E.coli) ;Recombinant Human TIM (N-6His, E.coli); Triosephosphate Isomerase; TIM; Triose-Phosphate Isomerase; TPI1; TPI |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C:3-6个月-80°C:12个月 |
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