Recombinant Human TF (C-6His, HEK293)是人类组织因子（TF）重组蛋白，在HEK293人源细胞中表达，带有C端6×His标签。该蛋白是单次跨膜I型糖蛋白，属于组织因子家族，其表达具有细胞类型依赖性，能介导细胞启动凝血级联反应，作为凝血因子VII的高亲和力受体，通过与VII/VIIa形成复合物激活IX/X因子，参与正常止血过程。
Human TF (C-6His, HEK293)/人TF蛋白(C-6His, HEK293) 化学结构 CAS号：N/A
Human TF (C-6His, HEK293)/人TF蛋白(C-6His, HEK293) 3D分子结构 CAS号：N/A
Human TF (C-6His, HEK293)/人TF蛋白(C-6His, HEK293) 质控信息
Human TF (C-6His, HEK293)/人TF蛋白(C-6His, HEK293) 生物活性
描述
Tissue Factor (TF) is a single-pass type I membrane glycoprotein member of the tissue factor family. TF expression is highly dependent upon cell type. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. TF initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human TF (C-6His, HEK293)/人TF蛋白(C-6His, HEK293) 技术信息
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