Recombinant Human Siglec-5 (C-Fc, HEK293)是人类来源的重组蛋白，在HEK293人源细胞中表达，带有C端Fc标签。该蛋白属于I型Ig类凝集素，是Ig超家族成员，其N端Ig样V区介导唾液酸结合，主要表达于单核/髓系细胞及外周血白细胞、脾脏、骨髓等组织，参与免疫细胞活化调控，与CD33相关Siglec家族共享高度序列相似性及免疫受体酪氨酸抑制基序（ITIMs）。
Human Siglec-5 (C-Fc, HEK293)/人Siglec-5蛋白(C-Fc, HEK293) 化学结构 CAS号：N/A
Human Siglec-5 (C-Fc, HEK293)/人Siglec-5蛋白(C-Fc, HEK293) 3D分子结构 CAS号：N/A
Human Siglec-5 (C-Fc, HEK293)/人Siglec-5蛋白(C-Fc, HEK293) 质控信息
Human Siglec-5 (C-Fc, HEK293)/人Siglec-5蛋白(C-Fc, HEK293) 生物活性
描述
Human Siglec-5 are Itype(Igtype) lectins belonging to the Ig superfamily,They are characterized by an N terminal Ig-like V type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2 type domains. SIGLEC5 has also been designated CD170, they are expressed by monocytic or myeloid lineage cells, and also found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. SIGLEC5 are expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation. Siglec5 to 11 share a high degree of sequence similarity with CD33/Siglec3 both in their extracellular and intracellular regions. They are collectively referred to as CD33 related Siglecs. One remarkable feature of the CD33 related Siglecs is their differential expression pattern within the hematopoietic system This fact, together with the presence of two conserved immunoreceptor tyrosinebased inhibition motifs (ITIMs) in their cytoplasma tails, suggests that CD33 related Siglecs are involved in the regulation of cellular activation within the immune system.
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150 mM NaCl, pH 8.0.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human Siglec-5 (C-Fc, HEK293)/人Siglec-5蛋白(C-Fc, HEK293) 技术信息
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