Recombinant Human Siglec-3 (C-mFc, HEK293)是人类来源的重组蛋白，在HEK293人源细胞中表达，带有C端mFc标签。该蛋白为CD33（Siglec-3），属于I型凝集素，是Ig超家族成员，其胞外区包含唾液酸结合结构域及免疫受体酪氨酸抑制基序（ITIMs），主要在髓系细胞表达，参与调控免疫细胞活化，可通过招募SHP1和SHP2负调免疫信号。
Human Siglec-3 (C-mFc, HEK293)/人Siglec-3蛋白(C-mFc, HEK293) 化学结构 CAS号：N/A
Human Siglec-3 (C-mFc, HEK293)/人Siglec-3蛋白(C-mFc, HEK293) 3D分子结构 CAS号：N/A
Human Siglec-3 (C-mFc, HEK293)/人Siglec-3蛋白(C-mFc, HEK293) 质控信息
Human Siglec-3 (C-mFc, HEK293)/人Siglec-3蛋白(C-mFc, HEK293) 生物活性
描述
CD33 is a type I Lectin belonging to the Ig superfamily. CD33 contains an N terminal Ig like V type domain, which mediates sialic acid binding, followed by one Ig like C2 type domain, a transmembrane region and a cytoplasmic tail containing two conserved immunoreceptor tyrosine based inhibition motifs (ITIMs). Eleven human Siglecs have been characterized. Siglecs 5 to 11 share a high degree of sequence similarity with CD33/Siglec3 both in their extracellular and intracellular regions. They are collectively referred to as CD33 related Siglecs. CD33 related Siglecs have differential expression pattern within the hematopoietic system. They are involved in the regulation of cellular activation within the immune system. Siglec 3 expression is restricted to cells of myelomonocytic lineage. Siglec3 recruits SHP1 and SHP2 to its ITIMs upon phosphorylation.
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human Siglec-3 (C-mFc, HEK293)/人Siglec-3蛋白(C-mFc, HEK293) 技术信息
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