Recombinant Human Siglec-2 (C-6His, HEK293)(Asp20-Arg687)是人类来源的重组蛋白，在HEK293细胞中表达，带有C端6×His标签。该蛋白为Siglec家族成员，又称CD22或BL-CAM，是B细胞限制性糖蛋白，主要表达于成熟B细胞表面及祖B、前B细胞的胞质中，能够优先结合α2,6-连接的唾液酸，介导细胞间黏附与信号调控。
Human Siglec-2 (C-6His, HEK293)(Asp20-Arg687) 化学结构 CAS号：N/A
Human Siglec-2 (C-6His, HEK293)(Asp20-Arg687) 3D分子结构 CAS号：N/A
Human Siglec-2 (C-6His, HEK293)(Asp20-Arg687) 质控信息
Human Siglec-2 (C-6His, HEK293)(Asp20-Arg687) 生物活性
描述
Siglecs (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins belonging to the Ig superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by varying numbers of Ig-like C2-type domains. Human Siglec-2, also known as B-cell antigen CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a B-cell restricted glycoprotein that is expressed in the cytoplasm of progenitor B and pre-B cells and on the surface of mature B cells. Two distinct human Siglec-2/CD22 cDNAs that arise from differential RNA processing of the same gene have been isolated. Siglec-2/CD22 is an adhesion molecule that preferentially binds alpha 2,6- linked sialic acid on the same (cis) or adjacent (trans) cells. Interaction of CD22 with trans ligands on opposing cells was found to be favored over the binding of ligands in cis.
Lyophilized from a 0.2 μm filtered solution of PBS, 1mM EDTA, pH7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human Siglec-2 (C-6His, HEK293)(Asp20-Arg687) 技术信息
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