Human Renin (C-10His, HEK293)/人Renin蛋白(C-10His, HEK293)
{[allProObj[0].p_purity_real_show]}
货号：A2925272同义名：
重组人Renin蛋白(C-10His, HEK293)
/ Recombinant Human Renin (C-10His, HEK293); Renin
Recombinant Human Renin (C-10His, HEK293)是人类重组肾素蛋白，在HEK293人源细胞中表达，带有C端10×His标签。该蛋白属于天冬氨酸蛋白酶家族，主要由肾脏球旁细胞产生，其前体为含43个N端脯氨酸残基的prorenin，经蛋白酶激活后具有高度底物选择性，可特异性切割血管紧张素原生成血管紧张素I，进而调控血压与盐平衡。
Human Renin (C-10His, HEK293)/人Renin蛋白(C-10His, HEK293) 化学结构 CAS号：N/A
Human Renin (C-10His, HEK293)/人Renin蛋白(C-10His, HEK293) 3D分子结构 CAS号：N/A
Human Renin (C-10His, HEK293)/人Renin蛋白(C-10His, HEK293) 质控信息
Human Renin (C-10His, HEK293)/人Renin蛋白(C-10His, HEK293) 生物活性
描述
Renin is a member of the aspartyl proteinase family produced largely in part by the juxtaglomerular cells in the kidney. Renin is produced as prorenin with 43 pro residues at the N-terminal of mature Renin. The inactive prorenin becomes activated proteolytically by trypsin, cathepsin B, or other proteinases. Renin also has a very high selectivity for substrates due to a long peptide recognition on either side of the peptide bond undergoing cleavage. An octapeptide substrate was the minimum length to be cleaved by Renin. Renin plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen,which is the only known physiological substrate of Renin. Renin releases the decapeptide angiotensin I, which in turn is further converted to vasoactive hormone angiotensin II by angiotensin converting enzyme (ACE).
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 8% Sucrose, 5% Mannitol, 0.05% Tween80, 100mM NaCl, pH 7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human Renin (C-10His, HEK293)/人Renin蛋白(C-10His, HEK293) 技术信息
CAS号
N/A
分子量
44 KDa
SMILES Code
NONE
别名
重组人Renin蛋白(C-10His, HEK293)
;Recombinant Human Renin (C-10His, HEK293); Renin; Angiotensinogenase; REN
运输
蓝冰
存储条件
In solvent-20°C:3-6个月-80°C:12个月
Pure formSealed in dry, store in freezer, under -20°C
搜索
