Recombinant Human ROR1 (C-6His, HEK293)是人源ROR1蛋白，在HEK293细胞中表达，带有C端6×His标签。该蛋白属于受体酪氨酸激酶样孤儿受体家族，是一种I型跨膜蛋白，全长937个氨基酸，包含信号肽、胞外域、跨膜区和胞内区。主要在心脏、肺和肾脏中高表达，参与WNT信号调控，可能作为WNT5A的受体发挥作用。
Human ROR1 (C-6His, HEK293)/人ROR1蛋白(C-6His, HEK293) 化学结构 CAS号：N/A
Human ROR1 (C-6His, HEK293)/人ROR1蛋白(C-6His, HEK293) 3D分子结构 CAS号：N/A
Human ROR1 (C-6His, HEK293)/人ROR1蛋白(C-6His, HEK293) 质控信息
Human ROR1 (C-6His, HEK293)/人ROR1蛋白(C-6His, HEK293) 生物活性
描述
Receptor tyrosine kinase-like orphan receptor 1 (ROR1), also known as neurotrophic tyrosine kinase, it is a member of the ROR family within receptor tyrosine kinases (RTK) superfamily. Human ROR1 is a type I transmembrane protein with 937 amino acids (aa) in length. It contains a 29 aa signal sequence, a 377 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 510 aa cytoplasmic region. ROR1 expressed strongly in human heart, lung and kidney, but weakly in the CNS. At developmental stage, it expressed at high levels during early embryonic development. ROR1 has been shown to have very low kinase activity in vitro and is unlikely to function as a tyrosine kinase in vivo. It may act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A-mediated signaling.
Lyophilized from a 0.2 μm filtered solution of 20mM Citrate, 5% Trehalose, 1mM EDTA, 0.02% Tween80, pH5.5.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human ROR1 (C-6His, HEK293)/人ROR1蛋白(C-6His, HEK293) 技术信息
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