Recombinant Human PECAM-1 (C-Fc, HEK293)是人源蛋白，在HEK293人源细胞中表达，带有C-Fc标签。该蛋白为血小板内皮细胞黏附分子（PECAM1/CD31），属于免疫球蛋白超家族，参与细胞黏附及白细胞跨内皮迁移，其胞外域介导同源相互作用，胞内域含ITIM基序，可调节免疫应答并维持外周耐受。
Human PECAM-1 (C-Fc, HEK293)/人PECAM-1蛋白(C-Fc, HEK293) 化学结构 CAS号：N/A
Human PECAM-1 (C-Fc, HEK293)/人PECAM-1蛋白(C-Fc, HEK293) 3D分子结构 CAS号：N/A
Human PECAM-1 (C-Fc, HEK293)/人PECAM-1蛋白(C-Fc, HEK293) 质控信息
Human PECAM-1 (C-Fc, HEK293)/人PECAM-1蛋白(C-Fc, HEK293) 生物活性
描述
Platelet Endothelial Cell Adhesion Molecule (PECAM1, CD31), is a heavily glycosylated transmembrane protein belonging to the immunoglobulin (Ig) superfamily of cell adhesion molecules. CD31 is composed of an extracellular domain (ECD) of 574 amino acids (aa) containing six Ig-like domains, a transmembrane domain, and a 118 aa cytoplasmic domain. CD31 is highly expressed on endothelial cells and at a lower level on platelets, granulocytes, macrophages, dendritic cells, T and B cells, and natural killer (NK) cells. It is involved in cell adhesion and is required for transepithelial migration of leukocytes (TEM). CD31 acts as a homophilic receptor through its extracellular domain and is involved in downstream signaling via its cytoplasmic domain. This domain contains highly conserved ITIM motifs which, once tyrosine phosphorylated, recruit and activate the signaling molecules Src and SHP2. The resulting inhibition of TCR signaling increases the activation threshold of T cells, thus reinforcing peripheral tolerance and preventing development of autoimmunity. CD31 additionally regulates immune responses by acting as a key inhibitory receptor in dendritic cell development. CD31 is required for the transendothelial migration of leukocytes through intercellular junctions of vascular endothelial cells.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human PECAM-1 (C-Fc, HEK293)/人PECAM-1蛋白(C-Fc, HEK293) 技术信息
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