Recombinant Human PDGFRA (C-6His, HEK293)是人类血小板衍生生长因子受体α蛋白，在人源细胞中表达，带有C端6×His标签。该蛋白属于受体酪氨酸激酶III类亚家族，是一种I型跨膜糖蛋白，可介导PDGF信号通路，参与调控神经嵴、心脏及肾脏间充质发育，并在炎症与组织培养诱导下表达上调，激活PI3K、Ras-MAPK及PLC-γ信号通路。
Human PDGFRA (C-6His, HEK293)/人PDGFRA蛋白(C-6His, HEK293) 化学结构 CAS号：N/A
Human PDGFRA (C-6His, HEK293)/人PDGFRA蛋白(C-6His, HEK293) 3D分子结构 CAS号：N/A
Human PDGFRA (C-6His, HEK293)/人PDGFRA蛋白(C-6His, HEK293) 质控信息
Human PDGFRA (C-6His, HEK293)/人PDGFRA蛋白(C-6His, HEK293) 生物活性
描述
Platelet-derived Growth Factor Receptor Alpha (PDGF Rα) is an enzyme that belongs to the class III subfamily of receptor tyrosine kinases.It is a type I transmembrane glycoprotein, and can form homo- or hetero-dimeric receptors when engaged by dimers of the PDGF family of growth factors, PDGF Rα is strongly expressed in oligodendrocyte, lung, skin and intestinal progenitor cells and induced by inflammation or growth in culture, but is lowly expressed in most mesenchymal cells. PDGF Rα autophosphorylates upon dimerization, activating signaling cascades in PI-3kinase Ras-MAP kinase, and PLC-γ pathways. PDGF Rα has infulence on local gradients of epithelially produced PDGF-AA or PDGF-CC during formation of the cranial ,cardiac neural crest and interstitial kidney mesenchyme.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human PDGFRA (C-6His, HEK293)/人PDGFRA蛋白(C-6His, HEK293) 技术信息
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