Human Neurturin (E.coli)/人Neurturin蛋白(Tag free, E.coli)
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货号：A2924036同义名：
重组人Neurturin蛋白(Tag free, E.coli)
/ Recombinant Human Neurturin (E.coli); Neurturin
Recombinant Human Neurturin (E.coli)是人源蛋白，在大肠杆菌中表达，为无标签形式。该蛋白属于GDNF家族配体，与GDNF、Persephin和Artemin同属，广泛表达于神经元和非神经元组织。其生物活性形式为羧基末端102个氨基酸组成的同源二聚体，通过结合GFRα-2（优先）或GFRα-1受体，并依赖cRET酪氨酸激酶传递信号，参与中枢与外周神经系统及非神经系统的发育与维持。
Human Neurturin (E.coli)/人Neurturin蛋白(Tag free, E.coli) 化学结构 CAS号：N/A
Human Neurturin (E.coli)/人Neurturin蛋白(Tag free, E.coli) 3D分子结构 CAS号：N/A
Human Neurturin (E.coli)/人Neurturin蛋白(Tag free, E.coli) 质控信息
Human Neurturin (E.coli)/人Neurturin蛋白(Tag free, E.coli) 生物活性
描述
Neurturin is a member of the GDNF family of ligands, which include glial cell-derived neurotrophic factor (GDNF), Neurturin, Persephin, and Artemin. Neurturin is expressed in both neuronal and nonneuronal tissues. Similarly to other TGFβ family proteins, Neurturin is synthesized as a precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxyterminal domain. The carboxy terminal domain of Neurturin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active human Neurturin is a disulfide-linked homodimer of the carboxy-terminal 102 amino acid residues. Unlike other members of TGF-β family, bioactivities of all GDNF family ligands are mediated through a unique multicomponent receptor complex composed of high affinity ligand binding component (GFRα-1-GFRα-4) and a common signaling component (cRET receptor tyrosine kinase). Each member of the GDNF family ligands has its preferred binding protein. Neurturin preferentially binds to GFRα-2 but can also bind GFRα-1 at higher concentrations. It may play a role in regulating the development and maintenance of the central and peripheral nervous systems and as well as non neuronal systems.
Lyophilized from a 0.2 μm filtered solution of 20mM Citrate, 6% Sucrose, 4% Mannitol, 0.05% Tween 80, pH 4.0.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human Neurturin (E.coli)/人Neurturin蛋白(Tag free, E.coli) 技术信息
CAS号
N/A
分子量
11.8 KDa
SMILES Code
NONE
别名
重组人Neurturin蛋白(Tag free, E.coli)
;Recombinant Human Neurturin (E.coli); Neurturin; NRTN
运输
蓝冰
存储条件
In solvent-20°C:3-6个月-80°C:12个月
Pure formSealed in dry, store in freezer, under -20°C
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