货号:A2924520
同义名:
重组人Nectin-3蛋白(C-6His, HEK293)
/ Recombinant Human Nectin-3 (C-6His, HEK293); Poliovirus Receptor-Related Protein 3
Recombinant Human Nectin-3 (C-6His, HEK293) 是人源 Nectin-3 蛋白,在 HEK293 细胞中表达,带有 C 端 6×His 标签。该蛋白是 I 型跨膜糖蛋白,属于 nectin 家族,参与细胞间黏附,通过与 nectin 或 nectin 样蛋白的异源相互作用,如与 PVRL2/Nectin-2 在支持细胞-精子结合处的作用,介导黏附连接和突触形成,主要表达于睾丸、胎盘及多种上皮细胞系。


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| 描述 | Nectin-3 is a type I transmembrane glycoprotein that belongs to the nectin family. Its precursor is 549 amino acids in length and contains an extended signal sequence of 57 amino acids, an extracellular domain (ECD) of 347 amino acids, a transmembrane segment of 21 amino acids, and a cytoplasmic region of 124 amino acids. It is predominantly expressed in testis and placenta as well as in various cell lines, including epithelial cell lines. Nectin-3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/Nectin-2 at Sertoli-spermatid junctions. Nectin-3 is also involved in the formation of cell-cell junctions, including adherens junctions and synapses. |
| 蛋白种属 | Human |
| 表达系统 | Human Cells |
| 标签 | C-6His |
| 蛋白表达区间 | Gly58-Cys366 |
| 蛋白编号 | Q9NQS3 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 干粉 |
| 剂型描述 | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 300mM NaCl, 5% Trehalose, pH 8.3. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。 分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 34.96 KDa |
| SMILES Code | NONE |
| 别名 | 重组人Nectin-3蛋白(C-6His, HEK293) ;Recombinant Human Nectin-3 (C-6His, HEK293); Poliovirus Receptor-Related Protein 3; CDw113; Nectin-3; CD113; PVRL3; PRR3 |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C: 3-6个月 -80°C: 12个月 Pure form Sealed in dry, store in freezer, under -20°C |
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