货号:A2925073
同义名:
重组人NT-proBNP蛋白(N-6His-Flag, E.coli)
/ Recombinant Human NT-proBNP (N-6His-Flag, E.coli); Natriuretic peptides B
Recombinant Human NT-proBNP (N-6His-Flag, E.coli)是人源蛋白,在大肠杆菌中表达,带有N端6His-Flag标签。该蛋白为脑钠肽(BNP)的N端前体片段,主要由心室肌细胞产生,属于利钠肽家族,是心力衰竭的重要生物标志物,其血清水平升高也可见于房颤及房间隔缺损等非心衰相关疾病。


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| 描述 | Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly by ventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12 kDa BNP precursor gives rise to N-terminal Pro BNP (NT-proBNP) and mature BNP. N-terminal proB-type natriuretic peptide (NT-proBNP), a useful marker of heart failure (HF), is considered to be secreted mainly from the ventricle, increased serum NT-proBNP levels are also encountered in conditions such as atrial fibrillation (AF) and atrial septal defect in patients without HF. |
| 蛋白种属 | Human |
| 表达系统 | E.coli |
| 标签 | N-6His-Flag |
| 蛋白表达区间 | His27-Arg102 |
| 蛋白编号 | P16860 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 干粉 |
| 剂型描述 | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。 分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 11 KDa |
| SMILES Code | NONE |
| 别名 | 重组人NT-proBNP蛋白(N-6His-Flag, E.coli) ;Recombinant Human NT-proBNP (N-6His-Flag, E.coli); Natriuretic peptides B; Gamma-brain natriuretic peptide; NPPB; BNP |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C: 3-6个月 -80°C: 12个月 Pure form Sealed in dry, store in freezer, under -20°C |
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