货号:A2900838 同义名:
重组人NAMPT蛋白(E.coli, N-6His)
/ Recombinant Human NAMPT (N-6His); Pre-B cell-enhancing factor
Recombinant Human NAMPT (N-6His)由大肠杆菌表达系统生产,目标基因编码Met1-His491,并在N末端带有6His标签。
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描述 | Pre-B cell colony enhancing factor (PBEF) was originally identified as a cytokine that potentiated the clonal expansion and differentiation of pre-B cells, but it is also acknowledged to be the ubiquitous intracellular enzyme nicotinamide phosphoribosyltranferase (NAMPT) and the adipokine “visfatin”. PBEF is constitutively expressed in the fetal membranes where its greatest expression is in the amnion. It has intracellular and extracellular forms. Most of the intracellular functions of PBEF are due to its role as a Nampt which can induce angiogenesis through upregulation of VEGF and VEGFR and secretion of MCP-1. Extracellular PBEF has been shown to increase inflammatory cytokines, such as TNF-α, IL-1β, IL-16, and TGF-β1. PBEF also increases the production of IL-6, TNF-α, and IL-1β in CD14+ monocyctes, macrophages, and dendritic cells, enhances the effectiveness of T cells. |
蛋白种属 | Human |
表达系统 | E.coli |
标签 | N-6His |
蛋白表达区间 | Met1-His491 |
蛋白编号 | P43490 |
内毒素 | < 1 EU/µg as determined by LAL test. |
剂型 | 干粉 |
剂型描述 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 10% Trehalose, 100mM Arg-HCl, 0.02% Tween 80, pH6.0. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
CAS号 | N/A |
分子量 | 57 KDa |
SMILES Code | NONE |
别名 | 重组人NAMPT蛋白(E.coli, N-6His) ;Recombinant Human NAMPT (N-6His); Pre-B cell-enhancing factor; Nicotinamide phosphoribosyltransferase; NAmPRTase; Nampt; Pre-B-cell colony-enhancing factor 1; Visfatin; NAMPT; PBEF; PBEF1 |
运输 | 蓝冰 |
存储条件 | In solvent -20°C: 3-6个月 -80°C: 12个月 Pure form Sealed in dry, store in freezer, under -20°C |